Generation by insulin of a chemical mediator that controls protein phosphorylation and dephosphorylation

J. Larner, G. Galasko, K. Cheng, A. A. DePaoli-Roach, L. Huang, P. Daggy, J. Kellogg

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Deproteinized skeletal muscle extracts free of major nudeotides from control and insulin-treated rats were fractionated and assayed for inhibition of protein phosphorylation by cyclic adenosine monophosphate (AMP)-dependent and -independent protein kinases. A differential effect of insulin on a particular fraction was observed on cyclic AMP-dependent protein kinase but not on cyclic AMP-independent protein kinases. This fraction that inhibited cyclic AMP-dependent protein kinase also stimulated glycogen synthase phosphoprotein phosphatase. It is proposed that this fraction may contain mediator substance generated in the presence of insulin.

Original languageEnglish (US)
Pages (from-to)1408-1410
Number of pages3
Issue number4425
StatePublished - Jan 1 1979


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