Generation by insulin of a chemical mediator that controls protein phosphorylation and dephosphorylation

J. Larner, G. Galasko, K. Cheng, Anna De Paoli-Roach, L. Huang, P. Daggy, J. Kellogg

Research output: Contribution to journalArticle

223 Citations (Scopus)

Abstract

Deproteinized skeletal muscle extracts free of major nudeotides from control and insulin-treated rats were fractionated and assayed for inhibition of protein phosphorylation by cyclic adenosine monophosphate (AMP)-dependent and -independent protein kinases. A differential effect of insulin on a particular fraction was observed on cyclic AMP-dependent protein kinase but not on cyclic AMP-independent protein kinases. This fraction that inhibited cyclic AMP-dependent protein kinase also stimulated glycogen synthase phosphoprotein phosphatase. It is proposed that this fraction may contain mediator substance generated in the presence of insulin.

Original languageEnglish (US)
Pages (from-to)1408-1410
Number of pages3
JournalScience
Volume206
Issue number4425
StatePublished - 1979
Externally publishedYes

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Cyclic AMP-Dependent Protein Kinases
Phosphorylation
Insulin
Glycogen Synthase
Proteins
Phosphoprotein Phosphatases
Cyclic AMP
Protein Kinases
Skeletal Muscle

ASJC Scopus subject areas

  • General

Cite this

Larner, J., Galasko, G., Cheng, K., De Paoli-Roach, A., Huang, L., Daggy, P., & Kellogg, J. (1979). Generation by insulin of a chemical mediator that controls protein phosphorylation and dephosphorylation. Science, 206(4425), 1408-1410.

Generation by insulin of a chemical mediator that controls protein phosphorylation and dephosphorylation. / Larner, J.; Galasko, G.; Cheng, K.; De Paoli-Roach, Anna; Huang, L.; Daggy, P.; Kellogg, J.

In: Science, Vol. 206, No. 4425, 1979, p. 1408-1410.

Research output: Contribution to journalArticle

Larner, J, Galasko, G, Cheng, K, De Paoli-Roach, A, Huang, L, Daggy, P & Kellogg, J 1979, 'Generation by insulin of a chemical mediator that controls protein phosphorylation and dephosphorylation', Science, vol. 206, no. 4425, pp. 1408-1410.
Larner, J. ; Galasko, G. ; Cheng, K. ; De Paoli-Roach, Anna ; Huang, L. ; Daggy, P. ; Kellogg, J. / Generation by insulin of a chemical mediator that controls protein phosphorylation and dephosphorylation. In: Science. 1979 ; Vol. 206, No. 4425. pp. 1408-1410.
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