Generation of a conditional null allele for Dmp1 in mouse

Jian Q. Feng, Greg Scott, Dayong Guo, Baichun Jiang, Marie Harris, Toni Ward, Manas Ray, Lynda F. Bonewald, Stephen E. Harris, Yuji Mishina

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Dentin matrix protein1 (DMP1), highly conserved in humans and mice, is highly expressed in teeth, the skeleton, and to a lesser extent in nonskeletal tissues such as brain, kidney, and salivary gland. Pathologically, DMP1 is associated with several forms of cancers and with tumor-induced osteomalacia. Conventional disruption of the murine Dmp1 gene results in defects in dentin in teeth and in the skeleton, including hypophosphatemic rickets, and abnormalities in phosphate homeostasis. Human DMP1 mutations are responsible for the condition known as autosomal recessive hypophosphatemic rickets. For better understanding of the roles of DMP1 in different tissues at different stages of development and in pathological conditions, we generated Dmp1 floxed mice in which loxP sites flank exon 6 that encodes for over 80% of DMP1 protein. We demonstrate that Cre-mediated recombination using Sox2-Cre, a Cre line expressed in epibiast during early embryogenesis, results in early deletion of the gene and protein. These homozygous Cre-recombined null mice display an identical phenotype to conventional null mice. This animal model will be useful to reveal distinct roles of DMP1 in different tissues at different ages.

Original languageEnglish (US)
Pages (from-to)87-91
Number of pages5
JournalGenesis
Volume46
Issue number2
DOIs
StatePublished - Feb 1 2008
Externally publishedYes

Keywords

  • Bone
  • Cre-loxP
  • DMP1
  • Gene targeting
  • Mouse

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

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  • Cite this

    Feng, J. Q., Scott, G., Guo, D., Jiang, B., Harris, M., Ward, T., Ray, M., Bonewald, L. F., Harris, S. E., & Mishina, Y. (2008). Generation of a conditional null allele for Dmp1 in mouse. Genesis, 46(2), 87-91. https://doi.org/10.1002/dvg.20370