Generation of functional hemangioblasts from human embryonic stem cells

Shi Jiang Lu, Qiang Feng, Sergio Caballero, Yu Chen, Malcolm A S Moore, Maria B. Grant, Robert Lanza

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

Recent evidence suggests the existence of progenitor cells in adult tissues that are capable of differentiating into vascular structures as well as into all hematopoietic cell lineages. Here we describe an efficient and reproducible method for generating large numbers of these bipotential progenitors-known as hemangioblasts-from human embryonic stem (hES) cells using an in vitro differentiation system. Blast cells expressed gene signatures characteristic of hemangioblasts, and could be expanded, cryopreserved and differentiated into multiple hematopoietic lineages as well as into endothelial cells. When we injected these cells into rats with diabetes or into mice with ischemia-reperfusion injury of the retina, they localized to the site of injury in the damaged vasculature and appeared to participate in repair. Injection of the cells also reduced the mortality rate after myocardial infarction and restored blood flow in hind limb ischemia in mouse models. Our data suggest that hES-derived blast cells (hES-BCs) could be important in vascular repair.

Original languageEnglish (US)
Pages (from-to)501-509
Number of pages9
JournalNature Methods
Volume4
Issue number6
DOIs
StatePublished - Jun 2007
Externally publishedYes

Fingerprint

Hemangioblasts
Stem cells
Repair
Endothelial cells
Medical problems
Blood Vessels
Rats
Blood
Genes
Tissue
Cell Lineage
Reperfusion Injury
Retina
Stem Cells
Ischemia
Extremities
Endothelial Cells
Myocardial Infarction
Injections
Mortality

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Cite this

Lu, S. J., Feng, Q., Caballero, S., Chen, Y., Moore, M. A. S., Grant, M. B., & Lanza, R. (2007). Generation of functional hemangioblasts from human embryonic stem cells. Nature Methods, 4(6), 501-509. https://doi.org/10.1038/nmeth1041

Generation of functional hemangioblasts from human embryonic stem cells. / Lu, Shi Jiang; Feng, Qiang; Caballero, Sergio; Chen, Yu; Moore, Malcolm A S; Grant, Maria B.; Lanza, Robert.

In: Nature Methods, Vol. 4, No. 6, 06.2007, p. 501-509.

Research output: Contribution to journalArticle

Lu, SJ, Feng, Q, Caballero, S, Chen, Y, Moore, MAS, Grant, MB & Lanza, R 2007, 'Generation of functional hemangioblasts from human embryonic stem cells', Nature Methods, vol. 4, no. 6, pp. 501-509. https://doi.org/10.1038/nmeth1041
Lu SJ, Feng Q, Caballero S, Chen Y, Moore MAS, Grant MB et al. Generation of functional hemangioblasts from human embryonic stem cells. Nature Methods. 2007 Jun;4(6):501-509. https://doi.org/10.1038/nmeth1041
Lu, Shi Jiang ; Feng, Qiang ; Caballero, Sergio ; Chen, Yu ; Moore, Malcolm A S ; Grant, Maria B. ; Lanza, Robert. / Generation of functional hemangioblasts from human embryonic stem cells. In: Nature Methods. 2007 ; Vol. 4, No. 6. pp. 501-509.
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