Generation of highly enriched populations of optic vesicle-like retinal cells from human pluripotent stem cells

Sarah K. Ohlemacher, Clara L. Iglesias, Akshayalakshmi Sridhar, David M. Gamm, Jason S. Meyer

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

The protocol outlined below is used to differentiate human pluripotent stem cells (hPSCs) into retinal cell types through a process that faithfully recapitulates the stepwise progression observed in vivo. From pluripotency, cells are differentiated to a primitive anterior neural fate, followed by progression into two distinct populations of retinal progenitors and forebrain progenitors, each of which can be manually separated and purified. The hPSC-derived retinal progenitors are found to self-organize into three-dimensional optic vesicle-like structures, with each aggregate possessing the ability to differentiate into all major retinal cell types. The ability to faithfully recapitulate the stepwise in vivo development in a three-dimensional cell culture system allows for the study of mechanisms underlying human retinogenesis. Furthermore, this methodology allows for the study of retinal dysfunction and disease modeling using patient-derived cells, as well as high-throughput pharmacological screening and eventually patient-specific therapies.

Original languageEnglish (US)
Pages (from-to)1h.8.1-1h.8.20
JournalCurrent Protocols in Stem Cell Biology
Volume2015
DOIs
StatePublished - 2015

Keywords

  • Development
  • Differentiation
  • Human pluripotent stem cells (hPSCs)
  • Optic vesicle
  • Retina

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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