Generation of pluripotent stem cells from neonatal mouse testis

Mito Kanatsu-Shinohara, Kimiko Inoue, Jiyoung Lee, Momoko Yoshimoto, Narumi Ogonuki, Hiromi Miki, Shiro Baba, Takeo Kato, Yasuhiro Kazuki, Shinya Toyokuni, Megumi Toyoshima, Ohtsura Niwa, Mitsuo Oshimura, Toshio Heike, Tatsutoshi Nakahata, Fumitoshi Ishino, Atsuo Ogura, Takashi Shinohara

Research output: Contribution to journalArticle

622 Citations (Scopus)

Abstract

Although germline cells can form multipotential embryonic stem (ES)/embryonic germ (EG) cells, these cells can be derived only from embryonic tissues, and such multipotent cells have not been available from neonatal gonads. Here we report the successful establishment of ES-like cells from neonatal mouse testis. These ES-like cells were phenotypically similar to ES/EG cells except in their genomic imprinting pattern. They differentiated into various types of somatic cells in vitro under conditions used to induce the differentiation of ES cells and produced teratomas after inoculation into mice. Furthermore, these ES-like cells formed germline chimeras when injected into blastocysts. Thus, the capacity to form multipotent cells persists in neonatal testis. The ability to derive multipotential stem cells from the neonatal testis has important implications for germ cell biology and opens the possibility of using these cells for biotechnology and medicine.

Original languageEnglish (US)
Pages (from-to)1001-1012
Number of pages12
JournalCell
Volume119
Issue number7
DOIs
StatePublished - Dec 29 2004
Externally publishedYes

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Pluripotent Stem Cells
Stem cells
Testis
Cytology
Embryonic Stem Cells
Biotechnology
Medicine
Cells
Tissue
Genomic Imprinting
Teratoma
Gonads
Blastocyst
Germ Cells
Cell Biology
Stem Cells

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Kanatsu-Shinohara, M., Inoue, K., Lee, J., Yoshimoto, M., Ogonuki, N., Miki, H., ... Shinohara, T. (2004). Generation of pluripotent stem cells from neonatal mouse testis. Cell, 119(7), 1001-1012. https://doi.org/10.1016/j.cell.2004.11.011

Generation of pluripotent stem cells from neonatal mouse testis. / Kanatsu-Shinohara, Mito; Inoue, Kimiko; Lee, Jiyoung; Yoshimoto, Momoko; Ogonuki, Narumi; Miki, Hiromi; Baba, Shiro; Kato, Takeo; Kazuki, Yasuhiro; Toyokuni, Shinya; Toyoshima, Megumi; Niwa, Ohtsura; Oshimura, Mitsuo; Heike, Toshio; Nakahata, Tatsutoshi; Ishino, Fumitoshi; Ogura, Atsuo; Shinohara, Takashi.

In: Cell, Vol. 119, No. 7, 29.12.2004, p. 1001-1012.

Research output: Contribution to journalArticle

Kanatsu-Shinohara, M, Inoue, K, Lee, J, Yoshimoto, M, Ogonuki, N, Miki, H, Baba, S, Kato, T, Kazuki, Y, Toyokuni, S, Toyoshima, M, Niwa, O, Oshimura, M, Heike, T, Nakahata, T, Ishino, F, Ogura, A & Shinohara, T 2004, 'Generation of pluripotent stem cells from neonatal mouse testis', Cell, vol. 119, no. 7, pp. 1001-1012. https://doi.org/10.1016/j.cell.2004.11.011
Kanatsu-Shinohara M, Inoue K, Lee J, Yoshimoto M, Ogonuki N, Miki H et al. Generation of pluripotent stem cells from neonatal mouse testis. Cell. 2004 Dec 29;119(7):1001-1012. https://doi.org/10.1016/j.cell.2004.11.011
Kanatsu-Shinohara, Mito ; Inoue, Kimiko ; Lee, Jiyoung ; Yoshimoto, Momoko ; Ogonuki, Narumi ; Miki, Hiromi ; Baba, Shiro ; Kato, Takeo ; Kazuki, Yasuhiro ; Toyokuni, Shinya ; Toyoshima, Megumi ; Niwa, Ohtsura ; Oshimura, Mitsuo ; Heike, Toshio ; Nakahata, Tatsutoshi ; Ishino, Fumitoshi ; Ogura, Atsuo ; Shinohara, Takashi. / Generation of pluripotent stem cells from neonatal mouse testis. In: Cell. 2004 ; Vol. 119, No. 7. pp. 1001-1012.
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AU - Miki, Hiromi

AU - Baba, Shiro

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AU - Toyokuni, Shinya

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AU - Niwa, Ohtsura

AU - Oshimura, Mitsuo

AU - Heike, Toshio

AU - Nakahata, Tatsutoshi

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AU - Ogura, Atsuo

AU - Shinohara, Takashi

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