Generation of Smad7-Cre recombinase mice

A useful tool for the study of epithelial-mesenchymal transformation within the embryonic heart

Paige Snider, Sunyong Tang, Goldie Lin, Jian Wang, Simon Conway

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Smad7 can be induced by various transforming growth factor-β superfamily ligands and negatively modulates their signaling, thus acting in a negative, autocrine feedback manner. Previous analyses have demonstrated that although Smad7 is widely expressed, it is predominantly found in the vascular endothelium. Because of the restricted spatiotemporal reporter expression driven via a novel 4.3 kb Smad7 promoter in endocardial cells overlying the hearts atrioventricular (AV) cushions; we hypothesized that a transgenic Cre line would prove useful for the analysis of endocardial cushion and valve formation. Here we describe a mouse line, Smad7Cre, where Cre is robustly expressed within both cardiac outflow and AV endocardial cushions. Additionally, as endocardial cells are thought to contribute at least in part to the formation of the endocardial cushion mesenchyme, we crossed the Smad7Cre mice to the ROSA26eGFP-DTA diphtheria toxin A-expressing mice in order to genetically ablate Smad7Cre expressing cells. Ablation of Smad7Cre cells resulted in embryonic lethality by E11.5 and largely acellular endocardial cushions.

Original languageEnglish
Pages (from-to)469-475
Number of pages7
JournalGenesis
Volume47
Issue number7
DOIs
StatePublished - 2009

Fingerprint

Endocardial Cushions
Epithelial-Mesenchymal Transition
Diphtheria Toxin
Vascular Endothelium
Transforming Growth Factors
Mesoderm
Ligands
Cre recombinase

Keywords

  • Cre recombinase
  • Cre/loxP cell ablation
  • Endocardial cushions
  • Heart development
  • Mouse embryo
  • Smad7

ASJC Scopus subject areas

  • Endocrinology
  • Cell Biology
  • Genetics

Cite this

Generation of Smad7-Cre recombinase mice : A useful tool for the study of epithelial-mesenchymal transformation within the embryonic heart. / Snider, Paige; Tang, Sunyong; Lin, Goldie; Wang, Jian; Conway, Simon.

In: Genesis, Vol. 47, No. 7, 2009, p. 469-475.

Research output: Contribution to journalArticle

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