Genetic interactions between hepatocyte nuclear factor-6 and notch signaling regulate mouse intrahepatic bile duct development in vivo

Charles Vanderpool, Erin E. Sparks, Kari A. Huppert, Maureen Gannon, Anna L. Means, Stacey S. Huppert

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Notch signaling and hepatocyte nuclear factor-6 (HNF-6) are two genetic factors known to affect lineage commitment in the bipotential hepatoblast progenitor cell (BHPC) population. A genetic interaction involving Notch signaling and HNF-6 in mice has been inferred through separate experiments showing that both affect BHPC specification and bile duct morphogenesis. To define the genetic interaction between HNF-6 and Notch signaling in an in vivo mouse model, we examined the effects of BHPC-specific loss of HNF-6 alone and within the background of BHPC-specific loss of recombination signal binding protein immunoglobulin kappa J (RBP-J), the common DNA-binding partner of all Notch receptors. Isolated loss of HNF-6 in this mouse model fails to demonstrate a phenotypic variance in bile duct development compared to control. However, when HNF-6 loss is combined with RBP-J loss, a phenotype consisting of cholestasis, hepatic necrosis, and fibrosis is observed that is more severe than the phenotype seen with Notch signaling loss alone. This phenotype is associated with significant intrahepatic biliary system abnormalities, including an early decrease in biliary epithelial cells, evolving to ductular proliferation and a decrease in the density of communicating peripheral bile duct branches. In this in vivo model, simultaneous loss of both HNF-6 and RBP-J results in down-regulation of both HNF-1β and Sox9 (sex determining region Y-related HMG box transcription factor 9). Conclusion: HNF-6 and Notch signaling interact in vivo to control expression of downstream mediators essential to the normal development of the intrahepatic biliary system. This study provides a model to investigate genetic interactions of factors important to intrahepatic bile duct development and their effect on cholestatic liver disease phenotypes.

Original languageEnglish (US)
Pages (from-to)233-243
Number of pages11
JournalHepatology
Volume55
Issue number1
DOIs
StatePublished - Jan 2012
Externally publishedYes

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Hepatocyte Nuclear Factor 6
Intrahepatic Bile Ducts
Stem Cells
Bile Ducts
Phenotype
Biliary Tract
Notch Receptors
Cholestasis
Morphogenesis
Genetic Recombination
Immunoglobulins
Liver Diseases
Carrier Proteins
Fibrosis
Transcription Factors
Necrosis
Down-Regulation
Epithelial Cells

ASJC Scopus subject areas

  • Hepatology

Cite this

Genetic interactions between hepatocyte nuclear factor-6 and notch signaling regulate mouse intrahepatic bile duct development in vivo. / Vanderpool, Charles; Sparks, Erin E.; Huppert, Kari A.; Gannon, Maureen; Means, Anna L.; Huppert, Stacey S.

In: Hepatology, Vol. 55, No. 1, 01.2012, p. 233-243.

Research output: Contribution to journalArticle

Vanderpool, Charles ; Sparks, Erin E. ; Huppert, Kari A. ; Gannon, Maureen ; Means, Anna L. ; Huppert, Stacey S. / Genetic interactions between hepatocyte nuclear factor-6 and notch signaling regulate mouse intrahepatic bile duct development in vivo. In: Hepatology. 2012 ; Vol. 55, No. 1. pp. 233-243.
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