Genetic variation in GPBAR1 predisposes to quantitative changes in colonic transit and bile acid excretion

Michael Camilleri, Andrea Shin, Irene Busciglio, Paula Carlson, Andres Acosta, Adil E. Bharucha, Duane Burton, Jesse Lamsam, Alan Lueke, Leslie J. Donato, Alan R. Zinsmeister

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The pathobiology of irritable bowel syndrome (IBS) is multifaceted. We aimed to identify candidate genes predisposing to quantitative traits in IBS. In 30 healthy volunteers, 30 IBS-constipation, and 64 IBS-diarrhea patients, we measured bowel symptoms, bile acid (BA) synthesis (serum 7α-hydroxy-4-cholesten-3-one and FGF19), fecal BA and fat, colonic transit (CT by scintigraphy), and intestinal permeability (IP by 2-sugar excretion). We assessed associations of candidate genes controlling BA metabolism (KLB rs17618244 and FGFR4 rs351855), BA receptor (GPBAR1 rs11554825), serotonin (5-HT) reuptake (SLC6A4 through rs4795541 which encodes for the 44-bp insert in 5HTTLPR), or immune activation (TNFSF15 rs4263839) with three primary quantitative traits of interest: colonic transit, BA synthesis, and fecal BA excretion. There were significant associations between fecal BA and CT at 48 h (r = 0.43; P < 0.001) and IP (r = 0.23; P = 0.015). GPBAR1 genotype was associated with CT48 (P = 0.003) and total fecal BA [P = 0.030, false detection rate (FDR) P = 0.033]. Faster CT48 observed with both CC and TT GPBAR1 genotypes was due to significant interaction with G allele of KLB, which increases BA synthesis and excretion. Other univariate associations (P < 0.05, without FDR correction) observed between GPBAR1 and symptom phenotype and gas sensation ratings support the role of GPBAR1 receptor. Associations between SLC6A4 and stool consistency, ease of passage, postprandial colonic tone, and total fecal BA excretion provide data in support of future hypothesis-testing studies. Genetic control of GPBAR1 receptor predisposing to pathobiological mechanisms in IBS provides evidence from humans in support of the importance of GPBAR1 to colonic motor and secretory functions demonstrated in animal studies.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume307
Issue number5
DOIs
StatePublished - Sep 1 2014
Externally publishedYes

Fingerprint

Bile Acids and Salts
Irritable Bowel Syndrome
Serotonin
Genotype
Constipation
Radionuclide Imaging
Genes
Diarrhea
Permeability
Healthy Volunteers
Gases
Fats
Alleles
Phenotype

Keywords

  • Bile acid
  • Immune
  • Serotonin
  • Transit

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology (medical)
  • Physiology
  • Hepatology

Cite this

Genetic variation in GPBAR1 predisposes to quantitative changes in colonic transit and bile acid excretion. / Camilleri, Michael; Shin, Andrea; Busciglio, Irene; Carlson, Paula; Acosta, Andres; Bharucha, Adil E.; Burton, Duane; Lamsam, Jesse; Lueke, Alan; Donato, Leslie J.; Zinsmeister, Alan R.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 307, No. 5, 01.09.2014.

Research output: Contribution to journalArticle

Camilleri, M, Shin, A, Busciglio, I, Carlson, P, Acosta, A, Bharucha, AE, Burton, D, Lamsam, J, Lueke, A, Donato, LJ & Zinsmeister, AR 2014, 'Genetic variation in GPBAR1 predisposes to quantitative changes in colonic transit and bile acid excretion', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 307, no. 5. https://doi.org/10.1152/ajpgi.00178.2014
Camilleri, Michael ; Shin, Andrea ; Busciglio, Irene ; Carlson, Paula ; Acosta, Andres ; Bharucha, Adil E. ; Burton, Duane ; Lamsam, Jesse ; Lueke, Alan ; Donato, Leslie J. ; Zinsmeister, Alan R. / Genetic variation in GPBAR1 predisposes to quantitative changes in colonic transit and bile acid excretion. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2014 ; Vol. 307, No. 5.
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