Genome-wide analysis of RAS/ERK signaling targets

Joshua P. Plotnik, Peter C. Hollenhorst

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Identifying gene expression changes mediated by signaling pathways is necessary to determine mechanisms that cause phenotypic change. Recent advances in next-generation sequencing and informatic pipelines have streamlined the ability for laboratories to create and analyze transcriptomic data. Here, we describe the preparation of samples and transcriptomic analysis in order to determine gene expression programs regulated by RAS/ERK signaling.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages277-288
Number of pages12
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1487
ISSN (Print)1064-3745

Keywords

  • Bioinformatics
  • Differential gene expression
  • Library preparation
  • Multiplex
  • RAS/ERK
  • RNA-seq
  • Transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Plotnik, J. P., & Hollenhorst, P. C. (2017). Genome-wide analysis of RAS/ERK signaling targets. In Methods in Molecular Biology (pp. 277-288). (Methods in Molecular Biology; Vol. 1487). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6424-6_21