Genome-wide analysis of RAS/ERK signaling targets

Joshua P. Plotnik, Peter Hollenhorst

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Identifying gene expression changes mediated by signaling pathways is necessary to determine mechanisms that cause phenotypic change. Recent advances in next-generation sequencing and informatic pipelines have streamlined the ability for laboratories to create and analyze transcriptomic data. Here, we describe the preparation of samples and transcriptomic analysis in order to determine gene expression programs regulated by RAS/ERK signaling.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages277-288
Number of pages12
Volume1487
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1487
ISSN (Print)10643745

Fingerprint

Genome
Gene Expression
Informatics

Keywords

  • Bioinformatics
  • Differential gene expression
  • Library preparation
  • Multiplex
  • RAS/ERK
  • RNA-seq
  • Transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Plotnik, J. P., & Hollenhorst, P. (2017). Genome-wide analysis of RAS/ERK signaling targets. In Methods in Molecular Biology (Vol. 1487, pp. 277-288). (Methods in Molecular Biology; Vol. 1487). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6424-6_21

Genome-wide analysis of RAS/ERK signaling targets. / Plotnik, Joshua P.; Hollenhorst, Peter.

Methods in Molecular Biology. Vol. 1487 Humana Press Inc., 2017. p. 277-288 (Methods in Molecular Biology; Vol. 1487).

Research output: Chapter in Book/Report/Conference proceedingChapter

Plotnik, JP & Hollenhorst, P 2017, Genome-wide analysis of RAS/ERK signaling targets. in Methods in Molecular Biology. vol. 1487, Methods in Molecular Biology, vol. 1487, Humana Press Inc., pp. 277-288. https://doi.org/10.1007/978-1-4939-6424-6_21
Plotnik JP, Hollenhorst P. Genome-wide analysis of RAS/ERK signaling targets. In Methods in Molecular Biology. Vol. 1487. Humana Press Inc. 2017. p. 277-288. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6424-6_21
Plotnik, Joshua P. ; Hollenhorst, Peter. / Genome-wide analysis of RAS/ERK signaling targets. Methods in Molecular Biology. Vol. 1487 Humana Press Inc., 2017. pp. 277-288 (Methods in Molecular Biology).
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