Genomic and proteomic analysis of the impact of mitotic quiescence on the engraftment of human CD34+ cells

Brahmananda Reddy Chitteti, Yunlong Liu, Edward Srour

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

It is well established that in adults, long-term repopulating hematopoietic stem cells (HSC) are mitotically quiescent cells that reside in specialized bone marrow (BM) niches that maintain the dormancy of HSC. Our laboratory demonstrated that the engraftment potential of human HSC (CD34+ cells) from BM and mobilized peripheral blood (MPB) is restricted to cells in the G0 phase of cell cycle but that in the case of umbilical cord blood (UCB) -derived CD34+ cells, cell cycle status is not a determining factor in the ability of these cells to engraft and sustain hematopoiesis. We used this distinct in vivo behavior of CD34+ cells from these tissues to identify genes associated with the engraftment potential of human HSC. CD34+ cells from BM, MPB, and UCB were fractionated into G0 and G1 phases of cell cycle and subjected in parallel to microarray and proteomic analyses. A total of 484 target genes were identified to be associated with engraftment potential of HSC. System biology modeling indicated that the top four signaling pathways associated with these genes are Integrin signaling, p53 signaling, cytotoxic T lymphocyte-mediated apoptosis, and Myc mediated apoptosis signaling. Our data suggest that a continuum of functions of hematopoietic cells directly associated with cell cycle progression may play a major role in governing the engraftment potential of stem cells. While proteomic analysis identified a total of 646 proteins in analyzed samples, a very limited overlap between genomic and proteomic data was observed. These data provide a new insight into the genetic control of engraftment of human HSC from distinct tissues and suggest that mitotic quiescence may not be the requisite characteristic of engrafting stem cells, but instead may be the physiologic status conducive to the expression of genetic elements favoring engraftment.

Original languageEnglish
Article numbere17498
JournalPLoS One
Volume6
Issue number3
DOIs
StatePublished - 2011

Fingerprint

Hematopoietic Stem Cells
Stem cells
Proteomics
proteomics
genomics
Cell Cycle
cell cycle
Cell Cycle Resting Phase
Blood
Cells
interphase
cells
bone marrow
Fetal Blood
Bone Marrow Cells
umbilical cord
Bone
Genes
blood
Stem Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Genomic and proteomic analysis of the impact of mitotic quiescence on the engraftment of human CD34+ cells. / Chitteti, Brahmananda Reddy; Liu, Yunlong; Srour, Edward.

In: PLoS One, Vol. 6, No. 3, e17498, 2011.

Research output: Contribution to journalArticle

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