Genomic DNA from mice: A comparison of recovery methods and tissue sources

John R. Hofstetter, Aiwu Zhang, Aimee R. Mayeda, Tim Guscar, John I. Nurnberger, Debomoy K. Lahiri

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Our aim is to identify an extraction method and the source of mouse tissue(s) that could allow a high-resolution genomic scan from a living mouse. We compared and optimized two methods for yield, purity of DNA, and their use in the polymerase chain reaction (PCR) of DNA extracted from different mouse tissues. In addition to whole blood, tissue samples from the brain liver, testis, and tail were included in this study. The Rapid Method (RM) is preferable for the whole blood samples and testis and brain tissue samples because it is quicker, less toxic, and more cost-effective than the proteinase K method (PM). For liver the PM produced higher yields of DNA with less degradation than the RM. For tail tip, the PM produced a higher yield of DNA, but the RM resulted in a higher yield of PCR product. From a living mouse, a tail snip generated a sufficient remount of DNA for several hundred PCRs but not a complete genomic scan. We suggest that the RM can be used to extract genomic DNA for a complete genomic scan which requires either testicular tissues or repeated blood samples from the suborbital sinus over several months without sacrificing the animal.

Original languageEnglish (US)
Pages (from-to)197-202
Number of pages6
JournalBiochemical and Molecular Medicine
Volume62
Issue number2
DOIs
StatePublished - Dec 1997

ASJC Scopus subject areas

  • Biochemistry

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