Genomic organization of the murine CTL lipase gene

Mark Kaplan, Sarah N. Boyer, Michael J. Grusby

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Murine cytotoxic T-lymphocyte (CTL) lipase was originally identified as an IL-4-inducible gene in CD8-positive T cells. To further our understanding of both the function and the regulation of CTL lipase in T cells, we have cloned and characterized the murine gene. Two overlapping phage clones spanning 29 kb contain the entire CTL lipase gene. The exon structure is similar to those characterized for the human and canine pancreatic lipase- related protein 1 genes, with notable differences in the 5' end. Transcripts initiate from a site that matches a consensus for an initiator sequence. Potential cis-regulatory elements in the CTL lipase 5' regulatory region that would confer dual tissue specificity in exocrine pancreas and cytotoxic T lymphocytes are identified. The implications of this promoter organization are discussed.

Original languageEnglish (US)
Pages (from-to)606-609
Number of pages4
JournalGenomics
Volume35
Issue number3
DOIs
StatePublished - Aug 1 1996
Externally publishedYes

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Cytotoxic T-Lymphocytes
Lipase
Genes
T-Lymphocytes
Exocrine Pancreas
Organ Specificity
Nucleic Acid Regulatory Sequences
Consensus Sequence
Interleukin-4
Bacteriophages
Canidae
Exons
Clone Cells

ASJC Scopus subject areas

  • Genetics

Cite this

Genomic organization of the murine CTL lipase gene. / Kaplan, Mark; Boyer, Sarah N.; Grusby, Michael J.

In: Genomics, Vol. 35, No. 3, 01.08.1996, p. 606-609.

Research output: Contribution to journalArticle

Kaplan, Mark ; Boyer, Sarah N. ; Grusby, Michael J. / Genomic organization of the murine CTL lipase gene. In: Genomics. 1996 ; Vol. 35, No. 3. pp. 606-609.
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