Genomic structure and characterization of the Drosophila S3 ribosomal/DNA repair gene and mutant alleles

Mark Kelley, Y. Xu, D. M. Wilson, W. A. Deutsch

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The Drosophila S3 protein is known to be associated with ribosomes, where it is thought to play a role in the initiation of protein translation. The S3 protein also contains a DNA repair activity, efficiently processing 8- oxoguanine residues in DNA via an N-glycosylase/apurinic-apyrimidinic (AP) lyase activity. The gene that encodes S3 has previously been localized to one of the Minute loci on chromosome 3 in Drosophila. This study focused on the genomic organization of S3 at M(3)95A, initial promoter characterization, and analysis of three mutant alleles at this locus. The S3 gene was found to be a single-copy gene 2 to 3 kb in length and containing a single intron. The upstream 1.6-kb region was analyzed for promoter activity, identifying a presumptive regulatory domain containing potential enhancer and suppressor elements. This finding is of interest, as the S3 gene is constitutively expressed throughout development and mRNA is most likely maternally inherited. Lastly, three Minute alleles from the same locus were sequenced and two alleles found to contain a 22-bp deletion in exon 2, resulting in a truncated S3 protein, although wildtype levels of S3 mRNA and protein were detected in the viable heterozygous Minute alleles, possibly reflecting dosage compensation.

Original languageEnglish
Pages (from-to)149-156
Number of pages8
JournalDNA and Cell Biology
Volume19
Issue number3
DOIs
StatePublished - 2000

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Ribosomal DNA
DNA Repair
Drosophila
Alleles
Genes
Translational Peptide Chain Initiation
Drosophila Proteins
Messenger RNA
Proteins
Lyases
Chromosomes, Human, Pair 3
Ribosomes
Introns
Exons
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Genomic structure and characterization of the Drosophila S3 ribosomal/DNA repair gene and mutant alleles. / Kelley, Mark; Xu, Y.; Wilson, D. M.; Deutsch, W. A.

In: DNA and Cell Biology, Vol. 19, No. 3, 2000, p. 149-156.

Research output: Contribution to journalArticle

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