Glass wool separation of rat polymorphonuclear leukocytes according to adherence and maturity

H. E. Broxmeyer, G. van Zant, E. F. Schultz, L. A. Koltun, J. LoBue, A. S. Gordon

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Combined autoradiographic and glass wool adherence procedures were used in an attempt to separate rat peripheral blood polymorphonuclear leukocytes (PMN) according to adherence and maturity. Tritium ( 3H) grain count labeling was utilized as a marker for PMN age based on the assumption and experimental evidence that, in normal animals, granulocytes first into the marrow granulocyte reserve are first out into the circulation. Five days after a pulse of 3H thymidine, the peripheral blood contained young PMN which were lightly labeled and older PMN which were more heavily labeled. The cellular fraction removed from glass wool columns with Earle's solution contained young (lightly labeled) non adherent PMN. The EDTA separated fraction contained young and old adherent PMN. Possible implications regarding PMN separation and transfusion to correct human granulocytopenic conditions are discussed.

Original languageEnglish (US)
Pages (from-to)118-124
Number of pages7
JournalRES Journal of the Reticuloendothelial Society
Volume18
Issue number2
StatePublished - Dec 1 1975
Externally publishedYes

ASJC Scopus subject areas

  • Hematology

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