Glucagon-like peptide-1 regulates cholecystokinin production in β-cells to protect from apoptosis

Amelia K. Linnemann, Joshua C. Neuman, Therese J. Battiola, Jaclyn A. Wisinski, Michelle E. Kimple, Dawn Belt Davis

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Cholecystokinin (CCK) is a classic gut hormone that is also expressed in the pancreatic islet, where it is highly up-regulated with obesity. Loss of CCK results in increased β-cell apoptosis in obese mice. Similarly, islet β-cells produce increased amounts of another gut peptide, glucagon-like peptide 1 (GLP-1), in response to cytokine and nutrient stimulation. GLP-1 also protects β-cells from apoptosis via cAMP-mediated mechanisms. Therefore, we hypothesized that the activation of islet-derived CCK and GLP-1 may be linked. We show here that both human and mouse islets secrete active GLP-1 as a function of body mass index/obesity. Furthermore, GLP-1 can rapidly stimulate β-cell CCK production and secretion through direct targeting by the cAMP-modulated transcription factor, cAMP response element binding protein (CREB). We find that cAMP-mediated signaling is required for Cck expression, but CCK regulation by cAMP does not require stimulatory levels of glucose or insulin secretion. We also show that CREB directly targets the Cck promoter in islets from obese (Leptinob/ob) mice. Finally, we demonstrate that the ability of GLP-1 to protect β-cells from cytokine-induced apoptosis is partially dependent on CCK receptor signaling. Taken together, our work suggests that in obesity, active GLP-1 produced in the islet stimulates CCK production and secretion in a paracrine manner via cAMP and CREB. This intraislet incretin loop may be one mechanism whereby GLP-1 protects β-cells from apoptosis.

Original languageEnglish (US)
Pages (from-to)978-987
Number of pages10
JournalMolecular Endocrinology
Volume29
Issue number7
DOIs
StatePublished - Jul 1 2015

Fingerprint

Glucagon-Like Peptide 1
Cholecystokinin
Apoptosis
Cyclic AMP Response Element-Binding Protein
Obesity
Islets of Langerhans
Glucagon-Like Peptides
Cytokines
Cholecystokinin Receptors
Incretins
Obese Mice
Body Mass Index
Transcription Factors
Hormones
Insulin
Glucose
Food
Peptides

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

Cite this

Glucagon-like peptide-1 regulates cholecystokinin production in β-cells to protect from apoptosis. / Linnemann, Amelia K.; Neuman, Joshua C.; Battiola, Therese J.; Wisinski, Jaclyn A.; Kimple, Michelle E.; Davis, Dawn Belt.

In: Molecular Endocrinology, Vol. 29, No. 7, 01.07.2015, p. 978-987.

Research output: Contribution to journalArticle

Linnemann, Amelia K. ; Neuman, Joshua C. ; Battiola, Therese J. ; Wisinski, Jaclyn A. ; Kimple, Michelle E. ; Davis, Dawn Belt. / Glucagon-like peptide-1 regulates cholecystokinin production in β-cells to protect from apoptosis. In: Molecular Endocrinology. 2015 ; Vol. 29, No. 7. pp. 978-987.
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