Glucocorticoid-induced autophagy in osteocytes

Xuechun Xia, Rekha Kar, Jelica Gluhak-Heinrich, Wei Yao, Nancy E. Lane, Lynda Bonewald, Sondip K. Biswas, Woo Kuen Lo, Jean X. Jiang

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Glucocorticoid (GC) therapy is the most frequent cause of secondary osteoporosis. In this study we have demonstrated that GC treatment induced the development of autophagy, preserving osteocyte viability. GC treatment resulted in an increase in autophagy markers and the accumulation of autophagosome vacuoles in vitro and in vivo promoted the onset of the osteocyte autophagy, as determined by expression of autophagy markers in an animal model of GC-induced osteoporosis. An autophagy inhibitor reversed the protective effects of GCs. The effects of GCs on osteocytes were in contrast to tumor necrosis factor ? (TNF-?), which induced apoptosis but not autophagy. Together this study reveals a novel mechanism for the effect of GC on osteocytes, shedding new insight into mechanisms responsible for bone loss in patients receiving GC therapy.

Original languageEnglish (US)
Pages (from-to)2479-2488
Number of pages10
JournalJournal of Bone and Mineral Research
Volume25
Issue number11
DOIs
StatePublished - Nov 2010
Externally publishedYes

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Osteocytes
Autophagy
Glucocorticoids
Osteoporosis
Therapeutics
Vacuoles
Animal Models
Tumor Necrosis Factor-alpha
Apoptosis
Bone and Bones

Keywords

  • autophagy
  • glucocorticoid
  • osteocyte
  • viability

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Xia, X., Kar, R., Gluhak-Heinrich, J., Yao, W., Lane, N. E., Bonewald, L., ... Jiang, J. X. (2010). Glucocorticoid-induced autophagy in osteocytes. Journal of Bone and Mineral Research, 25(11), 2479-2488. https://doi.org/10.1002/jbmr.160

Glucocorticoid-induced autophagy in osteocytes. / Xia, Xuechun; Kar, Rekha; Gluhak-Heinrich, Jelica; Yao, Wei; Lane, Nancy E.; Bonewald, Lynda; Biswas, Sondip K.; Lo, Woo Kuen; Jiang, Jean X.

In: Journal of Bone and Mineral Research, Vol. 25, No. 11, 11.2010, p. 2479-2488.

Research output: Contribution to journalArticle

Xia, X, Kar, R, Gluhak-Heinrich, J, Yao, W, Lane, NE, Bonewald, L, Biswas, SK, Lo, WK & Jiang, JX 2010, 'Glucocorticoid-induced autophagy in osteocytes', Journal of Bone and Mineral Research, vol. 25, no. 11, pp. 2479-2488. https://doi.org/10.1002/jbmr.160
Xia X, Kar R, Gluhak-Heinrich J, Yao W, Lane NE, Bonewald L et al. Glucocorticoid-induced autophagy in osteocytes. Journal of Bone and Mineral Research. 2010 Nov;25(11):2479-2488. https://doi.org/10.1002/jbmr.160
Xia, Xuechun ; Kar, Rekha ; Gluhak-Heinrich, Jelica ; Yao, Wei ; Lane, Nancy E. ; Bonewald, Lynda ; Biswas, Sondip K. ; Lo, Woo Kuen ; Jiang, Jean X. / Glucocorticoid-induced autophagy in osteocytes. In: Journal of Bone and Mineral Research. 2010 ; Vol. 25, No. 11. pp. 2479-2488.
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