Glucose control of rabbit skeletal muscle glycogenin expressed in COS cells

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Abstract

Glycogenin is a self-glucosylating protein involved in the initiation of glycogen synthesis. Rabbit skeletal muscle glycogenin, transiently expressed in COS cells, was found exclusively in the low speed supernatant fraction, with Mr 37-38,000. The protein was capable of self-glucosylation and was, if suitably primed, an effective substrate for glycogen synthase. Rabbit muscle glycogen synthase was similarly expressed, to a level 7-10-fold over the endogenous activity. Most of the expressed protein was found in the low speed pellet fraction. However, when co-expressed with glycogenin, a significant increase in the proportion of glycogen synthase in the soluble fraction was observed. Therefore, glycogenin interacts with glycogen synthase in the cell and redistributes the synthase to the soluble fraction. Co-expression of an inactive form of glycogenin did not affect glycogen synthase localization. The expressed glycogenin could be detected as two distinguishable species, differing slightly in electrophoretic mobility, depending on the glucose concentration of the cell culture medium. At 25 mM glucose, a form of Mr 38,000 was observed; however, upon transfer to 5 mM glucose, it converted to a species of slightly lower Mr. The Mr of the 38,000-dalton species could be also be reduced by treatment of the cell extract with α-amylase. It was additionally found that the 38,000-dalton glycogenin was a much more effective glycogen synthase substrate than the lower Mr species. These results, therefore, raise the possibility of a novel mechanism for the control of glycogen metabolism in which glucose levels would regulate the glucosylation state of glycogenin, which in turn would determine glycogenin's efficacy as a substrate for elongation by glycogen synthase.

Original languageEnglish
Pages (from-to)14701-14707
Number of pages7
JournalJournal of Biological Chemistry
Volume268
Issue number20
StatePublished - Jul 15 1993

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COS Cells
Glycogen Synthase
Muscle
Skeletal Muscle
Rabbits
Glucose
Glycogen
Substrates
glycogenin
Electrophoretic mobility
Proteins
Amylases
Cell Extracts
Cell culture
Metabolism
Culture Media
Elongation
Cell Culture Techniques
Cells
Muscles

ASJC Scopus subject areas

  • Biochemistry

Cite this

Glucose control of rabbit skeletal muscle glycogenin expressed in COS cells. / Skurat, Alexander; Cao, Youjia; Roach, Peter.

In: Journal of Biological Chemistry, Vol. 268, No. 20, 15.07.1993, p. 14701-14707.

Research output: Contribution to journalArticle

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N2 - Glycogenin is a self-glucosylating protein involved in the initiation of glycogen synthesis. Rabbit skeletal muscle glycogenin, transiently expressed in COS cells, was found exclusively in the low speed supernatant fraction, with Mr 37-38,000. The protein was capable of self-glucosylation and was, if suitably primed, an effective substrate for glycogen synthase. Rabbit muscle glycogen synthase was similarly expressed, to a level 7-10-fold over the endogenous activity. Most of the expressed protein was found in the low speed pellet fraction. However, when co-expressed with glycogenin, a significant increase in the proportion of glycogen synthase in the soluble fraction was observed. Therefore, glycogenin interacts with glycogen synthase in the cell and redistributes the synthase to the soluble fraction. Co-expression of an inactive form of glycogenin did not affect glycogen synthase localization. The expressed glycogenin could be detected as two distinguishable species, differing slightly in electrophoretic mobility, depending on the glucose concentration of the cell culture medium. At 25 mM glucose, a form of Mr 38,000 was observed; however, upon transfer to 5 mM glucose, it converted to a species of slightly lower Mr. The Mr of the 38,000-dalton species could be also be reduced by treatment of the cell extract with α-amylase. It was additionally found that the 38,000-dalton glycogenin was a much more effective glycogen synthase substrate than the lower Mr species. These results, therefore, raise the possibility of a novel mechanism for the control of glycogen metabolism in which glucose levels would regulate the glucosylation state of glycogenin, which in turn would determine glycogenin's efficacy as a substrate for elongation by glycogen synthase.

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