Glucose metabolism in vivo in four commonly used inbred mouse strains

Eric D. Berglund, Candice Y. Li, Greg Poffenberger, Julio E. Ayala, Patrick T. Fueger, Shannon E. Willis, Marybeth M. Jewell, Alvin C. Powers, David H. Wasserman

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

OBJECTIVE-To characterize differences in whole-body glucose metabolism between commonly used inbred mouse strains. RESEARCH DESIGN AND METHODS-Hyperinsulinemic-euglycemic (∼8.5 mmol/l) and -hypoglycemic (∼3.0 mmol/l) clamps were done in catheterized, 5-h-fasted mice to assess insulin action and hypoglycemic counter-regulatory responsiveness. Hyperglycemic clamps (∼15 mmol/l) were done to assess insulin secretion and compared with results in perifused islets. RESULTS-Insulin action and hypoglycemic counter-regulatory and insulin secretory phenotypes varied considerably in four inbred mouse strains. In vivo insulin secretion was greatest in 129X1/Sv mice, but the counter-regulatory response to hypogly- cemia was blunted. FVB/N mice in vivo showed no increase in glucose-stimulated insulin secretion, relative hepatic insulin resistance, and the highest counter-regulatory response to hypoglycemia. In DBA/2 mice, insulin action was lowest among the strains, and islets isolated had the greatest glucose-stimulated insulin secretion in vitro. In C57BL/6 mice, in vivo physiological responses to hyperinsulinemia at euglycemia and hypoglycemia were intermediate relative to other strains. Insulin secretion by C57BL/6 mice was similar to that in other strains in contrast to the blunted glucose-stimulated insulin secretion from isolated islets. CONCLUSIONS-Strain-dependent differences exist in four inbred mouse strains frequently used for genetic manipulation and study of glucose metabolism. These results are important for selecting inbred mice to study glucose metabolism and for interpreting and designing experiments.

Original languageEnglish (US)
Pages (from-to)1790-1799
Number of pages10
JournalDiabetes
Volume57
Issue number7
DOIs
StatePublished - Jul 2008
Externally publishedYes

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Inbred Strains Mice
Insulin
Glucose
Hypoglycemia
Hypoglycemic Agents
Inbred C57BL Mouse
Inbred DBA Mouse
Hyperinsulinism
Insulin Resistance
Research Design
Phenotype

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Berglund, E. D., Li, C. Y., Poffenberger, G., Ayala, J. E., Fueger, P. T., Willis, S. E., ... Wasserman, D. H. (2008). Glucose metabolism in vivo in four commonly used inbred mouse strains. Diabetes, 57(7), 1790-1799. https://doi.org/10.2337/db07-1615

Glucose metabolism in vivo in four commonly used inbred mouse strains. / Berglund, Eric D.; Li, Candice Y.; Poffenberger, Greg; Ayala, Julio E.; Fueger, Patrick T.; Willis, Shannon E.; Jewell, Marybeth M.; Powers, Alvin C.; Wasserman, David H.

In: Diabetes, Vol. 57, No. 7, 07.2008, p. 1790-1799.

Research output: Contribution to journalArticle

Berglund, ED, Li, CY, Poffenberger, G, Ayala, JE, Fueger, PT, Willis, SE, Jewell, MM, Powers, AC & Wasserman, DH 2008, 'Glucose metabolism in vivo in four commonly used inbred mouse strains', Diabetes, vol. 57, no. 7, pp. 1790-1799. https://doi.org/10.2337/db07-1615
Berglund ED, Li CY, Poffenberger G, Ayala JE, Fueger PT, Willis SE et al. Glucose metabolism in vivo in four commonly used inbred mouse strains. Diabetes. 2008 Jul;57(7):1790-1799. https://doi.org/10.2337/db07-1615
Berglund, Eric D. ; Li, Candice Y. ; Poffenberger, Greg ; Ayala, Julio E. ; Fueger, Patrick T. ; Willis, Shannon E. ; Jewell, Marybeth M. ; Powers, Alvin C. ; Wasserman, David H. / Glucose metabolism in vivo in four commonly used inbred mouse strains. In: Diabetes. 2008 ; Vol. 57, No. 7. pp. 1790-1799.
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AU - Poffenberger, Greg

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AU - Fueger, Patrick T.

AU - Willis, Shannon E.

AU - Jewell, Marybeth M.

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AU - Wasserman, David H.

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