Glucose regulated insulin biosynthesis in isolated rat pancreatic islets is accompanied by changes in proinsulin mRNA

S. J. Giddings, John Chirgwin, M. A. Permutt

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Isolated rat pancreatic islets were incubated in high (28 mM) or low (2.8 mM) glucose for 0, 1 or 4 hr and insulin biosynthesis and messenger RNA quantified. During the 4 hr course of the experiments the fraction of protein synthesised specifically into proinsulin increased in the presence of high vs low glucose (14.3 ± 3.7 vs 0.8 ± 0.5%, p <0.01). The relative amount of proinsulin mRNA was found to be about 5-fold higher in islets incubated in high glucose at 4 hr as determined by RNA blot hybridization. Total translatable islet mRNA was unaffected by glucose in the medium. These data extend observations of changes in proinsulin mRNA in rats in vivo during fasting and glucose injection, and suggest a direct effect of glucose on islets. These data further demonstrate that glucose modulates selectively the level of proinsulin mRNA during incubation of isolated pancreatic islets and that changes in the level of proinsulin mRNA play an important role in regulation of insulin biosynthesis.

Original languageEnglish (US)
Pages (from-to)71-75
Number of pages5
JournalDiabetes Research
Volume2
Issue number2
StatePublished - 1985
Externally publishedYes

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Proinsulin
Islets of Langerhans
Insulin
Glucose
Messenger RNA
Fasting
RNA
Injections

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Glucose regulated insulin biosynthesis in isolated rat pancreatic islets is accompanied by changes in proinsulin mRNA. / Giddings, S. J.; Chirgwin, John; Permutt, M. A.

In: Diabetes Research, Vol. 2, No. 2, 1985, p. 71-75.

Research output: Contribution to journalArticle

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