GLUT1 and p63 expression in endometrial intraepithelial and uterine serous papillary carcinoma

Muhammad Idrees, P. Schlosshauer, G. Li, D. E. Burstein

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Aims: To analyse the expression patterns of GLUT1, p63 and p53 and the possible correlation between these markers in uterine serous papillary carcinoma (USPC) and endometrial intraepithelial carcinoma (EIC). Methods and results: Fourteen cases of USPC, 12 of which also showed EIC, were examined for GLUT1, p63 and p53 immunoreactivity. Four-micrometre sections from formalin-fixed paraffin-embedded tissue were immunostained using commercially available primary antibodies and Dako Envision Plus reagents for visualization. Membranous GLUT1 immunoreactivity was observed in all cases, including all 14 invasive tumours (100%) and 11 of 12 associated EICs (92%), and was confined to neoplastic cells. In USPC, staining tended to be strongest in superficial antistromal regions. p63 positivity was found in 8/14 (57%) USPCs and 9/12 (75%) associated EICs. In 11 cases p53 was overexpressed in both invasive USPC (11/14; 79%) and EIC (11/12; 92%). p53+ USPCs tended to be positive for p63, whereas p53- USPCs were also negative for p63. Conclusions: GLUT1 is expressed in the vast majority of USPC and EIC, suggesting a biological role during the early steps of carcinogenesis in endometrial serous neoplasms. GLUT1 expression may be induced by hypoxia-related as well as other mechanisms.

Original languageEnglish (US)
Pages (from-to)75-81
Number of pages7
JournalHistopathology
Volume49
Issue number1
DOIs
StatePublished - Jul 2006
Externally publishedYes

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Papillary Carcinoma
Endometrial Neoplasms
Carcinoma in Situ
Paraffin
Formaldehyde
Carcinogenesis
Staining and Labeling
Antibodies
Neoplasms

Keywords

  • Carcinogenesis
  • Endometrial intraepithelial carcinoma
  • GLUT1
  • p63
  • Uterine serous papillary carcinoma

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine
  • Cell Biology

Cite this

GLUT1 and p63 expression in endometrial intraepithelial and uterine serous papillary carcinoma. / Idrees, Muhammad; Schlosshauer, P.; Li, G.; Burstein, D. E.

In: Histopathology, Vol. 49, No. 1, 07.2006, p. 75-81.

Research output: Contribution to journalArticle

Idrees, Muhammad ; Schlosshauer, P. ; Li, G. ; Burstein, D. E. / GLUT1 and p63 expression in endometrial intraepithelial and uterine serous papillary carcinoma. In: Histopathology. 2006 ; Vol. 49, No. 1. pp. 75-81.
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AB - Aims: To analyse the expression patterns of GLUT1, p63 and p53 and the possible correlation between these markers in uterine serous papillary carcinoma (USPC) and endometrial intraepithelial carcinoma (EIC). Methods and results: Fourteen cases of USPC, 12 of which also showed EIC, were examined for GLUT1, p63 and p53 immunoreactivity. Four-micrometre sections from formalin-fixed paraffin-embedded tissue were immunostained using commercially available primary antibodies and Dako Envision Plus reagents for visualization. Membranous GLUT1 immunoreactivity was observed in all cases, including all 14 invasive tumours (100%) and 11 of 12 associated EICs (92%), and was confined to neoplastic cells. In USPC, staining tended to be strongest in superficial antistromal regions. p63 positivity was found in 8/14 (57%) USPCs and 9/12 (75%) associated EICs. In 11 cases p53 was overexpressed in both invasive USPC (11/14; 79%) and EIC (11/12; 92%). p53+ USPCs tended to be positive for p63, whereas p53- USPCs were also negative for p63. Conclusions: GLUT1 is expressed in the vast majority of USPC and EIC, suggesting a biological role during the early steps of carcinogenesis in endometrial serous neoplasms. GLUT1 expression may be induced by hypoxia-related as well as other mechanisms.

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