Glutathione S-transferases

Two-dimensional electrophoretic protein markers of lead exposure

Frank Witzmann, Daniel A. Daggett, Carla D. Fultz, Shelli A. Nelson, Lynda S. Wright, Steven E. Komguth, Frank L. Siegel

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Glutathione S-transferases (GST) are a family of detoxification isoenzymes that catalyze the conjugation of xenobiotics and their metabolites with reduced glutathione. Lead exposure in rats is known to induce GST isoenzymes in the liver and kidney. These changes in expression have potential use as biomarkers of lead exposure. Because two-dimensional electrophoresis (2-DE) enables one to analyze both protein abundance changes and chemical changes in protein structure, 2-DE was used to determine the effect of in vivo lead exposure on GST isoform expression in rat kidney cytosols. Male Sprague-Dawley rats were exposed to inorganic lead, and proteins were separated by conventional ISO-DALT and NEPHGE-DALT techniques and blotted for immunological identification. Lead exposure caused detectable inductions in both GSTP1 and GSTM1 and quantifiable charge modification in GSTP1. These preliminary data confirm the utility of 2-D electrophoretic GST analysis as indicative of lead exposure and toxicity and support its use for further elaboration of lead's effects on renal protein expression.

Original languageEnglish (US)
Pages (from-to)1332-1335
Number of pages4
JournalElectrophoresis
Volume19
Issue number8-9
DOIs
StatePublished - Jun 1998

Fingerprint

Glutathione Transferase
Rats
Proteins
Kidney
Isoenzymes
Immunologic Techniques
Detoxification
Biomarkers
Xenobiotics
Metabolites
Electrophoresis
Liver
Cytosol
Glutathione
Sprague Dawley Rats
Toxicity
Lead
Protein Isoforms

Keywords

  • Glutathione S-transferase
  • Lead exposure
  • Polyacrylamide gel electrophoresis
  • Rat kidney
  • Two-dimensional nonequilibrium pH gradient gel electrophoresis

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Witzmann, F., Daggett, D. A., Fultz, C. D., Nelson, S. A., Wright, L. S., Komguth, S. E., & Siegel, F. L. (1998). Glutathione S-transferases: Two-dimensional electrophoretic protein markers of lead exposure. Electrophoresis, 19(8-9), 1332-1335. https://doi.org/10.1002/elps.1150190821

Glutathione S-transferases : Two-dimensional electrophoretic protein markers of lead exposure. / Witzmann, Frank; Daggett, Daniel A.; Fultz, Carla D.; Nelson, Shelli A.; Wright, Lynda S.; Komguth, Steven E.; Siegel, Frank L.

In: Electrophoresis, Vol. 19, No. 8-9, 06.1998, p. 1332-1335.

Research output: Contribution to journalArticle

Witzmann, F, Daggett, DA, Fultz, CD, Nelson, SA, Wright, LS, Komguth, SE & Siegel, FL 1998, 'Glutathione S-transferases: Two-dimensional electrophoretic protein markers of lead exposure', Electrophoresis, vol. 19, no. 8-9, pp. 1332-1335. https://doi.org/10.1002/elps.1150190821
Witzmann, Frank ; Daggett, Daniel A. ; Fultz, Carla D. ; Nelson, Shelli A. ; Wright, Lynda S. ; Komguth, Steven E. ; Siegel, Frank L. / Glutathione S-transferases : Two-dimensional electrophoretic protein markers of lead exposure. In: Electrophoresis. 1998 ; Vol. 19, No. 8-9. pp. 1332-1335.
@article{ff5099a157e14aea868f76dc21edd67b,
title = "Glutathione S-transferases: Two-dimensional electrophoretic protein markers of lead exposure",
abstract = "Glutathione S-transferases (GST) are a family of detoxification isoenzymes that catalyze the conjugation of xenobiotics and their metabolites with reduced glutathione. Lead exposure in rats is known to induce GST isoenzymes in the liver and kidney. These changes in expression have potential use as biomarkers of lead exposure. Because two-dimensional electrophoresis (2-DE) enables one to analyze both protein abundance changes and chemical changes in protein structure, 2-DE was used to determine the effect of in vivo lead exposure on GST isoform expression in rat kidney cytosols. Male Sprague-Dawley rats were exposed to inorganic lead, and proteins were separated by conventional ISO-DALT and NEPHGE-DALT techniques and blotted for immunological identification. Lead exposure caused detectable inductions in both GSTP1 and GSTM1 and quantifiable charge modification in GSTP1. These preliminary data confirm the utility of 2-D electrophoretic GST analysis as indicative of lead exposure and toxicity and support its use for further elaboration of lead's effects on renal protein expression.",
keywords = "Glutathione S-transferase, Lead exposure, Polyacrylamide gel electrophoresis, Rat kidney, Two-dimensional nonequilibrium pH gradient gel electrophoresis",
author = "Frank Witzmann and Daggett, {Daniel A.} and Fultz, {Carla D.} and Nelson, {Shelli A.} and Wright, {Lynda S.} and Komguth, {Steven E.} and Siegel, {Frank L.}",
year = "1998",
month = "6",
doi = "10.1002/elps.1150190821",
language = "English (US)",
volume = "19",
pages = "1332--1335",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "8-9",

}

TY - JOUR

T1 - Glutathione S-transferases

T2 - Two-dimensional electrophoretic protein markers of lead exposure

AU - Witzmann, Frank

AU - Daggett, Daniel A.

AU - Fultz, Carla D.

AU - Nelson, Shelli A.

AU - Wright, Lynda S.

AU - Komguth, Steven E.

AU - Siegel, Frank L.

PY - 1998/6

Y1 - 1998/6

N2 - Glutathione S-transferases (GST) are a family of detoxification isoenzymes that catalyze the conjugation of xenobiotics and their metabolites with reduced glutathione. Lead exposure in rats is known to induce GST isoenzymes in the liver and kidney. These changes in expression have potential use as biomarkers of lead exposure. Because two-dimensional electrophoresis (2-DE) enables one to analyze both protein abundance changes and chemical changes in protein structure, 2-DE was used to determine the effect of in vivo lead exposure on GST isoform expression in rat kidney cytosols. Male Sprague-Dawley rats were exposed to inorganic lead, and proteins were separated by conventional ISO-DALT and NEPHGE-DALT techniques and blotted for immunological identification. Lead exposure caused detectable inductions in both GSTP1 and GSTM1 and quantifiable charge modification in GSTP1. These preliminary data confirm the utility of 2-D electrophoretic GST analysis as indicative of lead exposure and toxicity and support its use for further elaboration of lead's effects on renal protein expression.

AB - Glutathione S-transferases (GST) are a family of detoxification isoenzymes that catalyze the conjugation of xenobiotics and their metabolites with reduced glutathione. Lead exposure in rats is known to induce GST isoenzymes in the liver and kidney. These changes in expression have potential use as biomarkers of lead exposure. Because two-dimensional electrophoresis (2-DE) enables one to analyze both protein abundance changes and chemical changes in protein structure, 2-DE was used to determine the effect of in vivo lead exposure on GST isoform expression in rat kidney cytosols. Male Sprague-Dawley rats were exposed to inorganic lead, and proteins were separated by conventional ISO-DALT and NEPHGE-DALT techniques and blotted for immunological identification. Lead exposure caused detectable inductions in both GSTP1 and GSTM1 and quantifiable charge modification in GSTP1. These preliminary data confirm the utility of 2-D electrophoretic GST analysis as indicative of lead exposure and toxicity and support its use for further elaboration of lead's effects on renal protein expression.

KW - Glutathione S-transferase

KW - Lead exposure

KW - Polyacrylamide gel electrophoresis

KW - Rat kidney

KW - Two-dimensional nonequilibrium pH gradient gel electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=0031861447&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031861447&partnerID=8YFLogxK

U2 - 10.1002/elps.1150190821

DO - 10.1002/elps.1150190821

M3 - Article

VL - 19

SP - 1332

EP - 1335

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 8-9

ER -