Glutathione sulfhydryl transferase π expression in testicular germ cell tumors: An immunohistochemical study of 30 cases

D. J. Grignon, W. Mertens

Research output: Contribution to journalArticle

Abstract

Glutathione sulfhydryl transferase (GST) is believed to play a major role in the detoxification of chemotherapeutic agents and therefore is thought to be important in chemoresistance. Thirty primary testicular germ cell tumors were stained immunohistochemically for GSTπ using a rabbit polyclonal antibody and formalin-fixed paraffin-embedded tissue. There were 12 pure seminomas and 18 mixed germ cell tumors (GCTs). Immunostaining of each element present was graded as negative (-), weakly positive (+), or strongly positive (++). The results were as follows: seminoma (8/17 +, 9/17 ++); embryonal carcinoma (1/13 -, 7/13 +, 5/13 ++); mature teratoma (9/9 ++); immature teratoma (7/7 +); endodermal sinus tumor (7/8 +, 1/8 ++); and choriocarcinoma (1/1 -). These results show that variability exists in the expression of GSTπ between different tumor types and between different examples of the same tumor type. Strongest expression was seen in seminomas and mature teratomas while other germ cell elements tended to show weaker staining. The staining patterns showed no apparent correlation with stage or response to therapy.

Original languageEnglish (US)
Pages (from-to)607-613
Number of pages7
JournalOncology reports
Volume2
Issue number4
StatePublished - Jan 1 1995

Keywords

  • Drug resistance
  • Germ cell tumor
  • Glutathione transferase
  • Testis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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