Glycan localization within the human interphotoreceptor matrix and photoreceptor inner and outer segments

Paul N. Bishop, Mike Boulton, David Mcleod, Robert W. Stoddart

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Glycoconjugates are likely to be of fundamental importance in the complex interactions between photoreceptors and the retinal pigment epithelium, but few have been characterized, especially in human tissue. As a preliminary step towards determining their biological functions in health and disease, a lectin-based histochemical study of the glycan expression of human outer retina was performed on glutaraldehyde-fixed, semi-thin, resin-embedded sections. The interphotoreceptor matrix and photoreceptor plasma-lemmata expressed complex bisected and non-bisected biantennary and/or triantennary N-glycans. In addition, both the rod and cone outer segments bound strongly Galanthus nivalis agglutinin (which binds terminal Manα1, 3Man-) and the rod outer segments bound selectively the isolectin II of Bandeiraea simplicifolia (which binds terminal GlcNAc-). The cilia of the rods and cones were labelled selectively with Glycine max agglutinin after sialidase pretreatment. Four putative glycan outer sequences were identified within the interphotoreceptor matrix: (i) sialylated glycans with subterminal GaINAcα1, 3GaINAc-sequences; (ii) a sialylated type with subterminal N-acetyl-lactosamine residues; (iii) Galβ1, 3GalNAcα1- residues which were substituted with sialic acid except in the cone matrix sheath; (iv) GalNAcα1, 6Galβ1- residues which were substituted in part with sialic acid. The sialic acid expression throughout was predominantly of the 2, 3-linked form with lesser amounts of 2, 6-linkage, and rod-associated structures (including the surrounding interphotoreceptor matrix) were labelled more strongly with the sialic acid-binding lectins than cone-associated structures (including the cone matrix sheath).

Original languageEnglish (US)
Pages (from-to)403-412
Number of pages10
JournalGlycobiology
Volume3
Issue number4
DOIs
StatePublished - Aug 1993
Externally publishedYes

Fingerprint

N-Acetylneuraminic Acid
Polysaccharides
Cones
Cone
Lectins
Rod Cell Outer Segment
Vertebrate Photoreceptor Cells
Lectin
Acids
Glycoconjugates
Retinal Pigment Epithelium
Cilia
Neuraminidase
Glutaral
Glycine Max
Retinal Pigments
Retina
Pigments
Linkage
Amino acids

Keywords

  • Interphotoreceptor matrix
  • Lectin
  • Oligosaccharide
  • Photoreceptor
  • Retina

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Neuropsychology and Physiological Psychology
  • Hematology
  • Biochemistry

Cite this

Glycan localization within the human interphotoreceptor matrix and photoreceptor inner and outer segments. / Bishop, Paul N.; Boulton, Mike; Mcleod, David; Stoddart, Robert W.

In: Glycobiology, Vol. 3, No. 4, 08.1993, p. 403-412.

Research output: Contribution to journalArticle

Bishop, Paul N. ; Boulton, Mike ; Mcleod, David ; Stoddart, Robert W. / Glycan localization within the human interphotoreceptor matrix and photoreceptor inner and outer segments. In: Glycobiology. 1993 ; Vol. 3, No. 4. pp. 403-412.
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