Glycogen Dynamics Drives Lipid Droplet Biogenesis during Brown Adipocyte Differentiation

Alicia Mayeuf-Louchart, Steve Lancel, Yasmine Sebti, Benoit Pourcet, Anne Loyens, Stéphane Delhaye, Christian Duhem, Justine Beauchamp, Lise Ferri, Quentin Thorel, Alexis Boulinguiez, Mathilde Zecchin, Julie Dubois-Chevalier, Jérôme Eeckhoute, Logan T. Vaughn, Peter J. Roach, Christian Dani, Bartholomew A. Pederson, Stéphane D. Vincent, Bart StaelsHélène Duez

Research output: Contribution to journalArticle

Abstract

Browning induction or transplantation of brown adipose tissue (BAT) or brown/beige adipocytes derived from progenitor or induced pluripotent stem cells (iPSCs) can represent a powerful strategy to treat metabolic diseases. However, our poor understanding of the mechanisms that govern the differentiation and activation of brown adipocytes limits the development of such therapy. Various genetic factors controlling the differentiation of brown adipocytes have been identified, although most studies have been performed using in vitro cultured pre-adipocytes. We investigate here the differentiation of brown adipocytes from adipose progenitors in the mouse embryo. We demonstrate that the formation of multiple lipid droplets (LDs) is initiated within clusters of glycogen, which is degraded through glycophagy to provide the metabolic substrates essential for de novo lipogenesis and LD formation. Therefore, this study uncovers the role of glycogen in the generation of LDs.

Original languageEnglish (US)
Pages (from-to)1410-1418.e6
JournalCell Reports
Volume29
Issue number6
DOIs
StatePublished - Nov 5 2019

Fingerprint

Brown Adipocytes
Glycogen
Lipids
Stem cells
Induced Pluripotent Stem Cells
Lipogenesis
Brown Adipose Tissue
Metabolic Diseases
Chemical activation
Adipocytes
Tissue
Embryonic Structures
Transplantation
Substrates
Lipid Droplets

Keywords

  • adipocyte differentiation
  • adipocyte metabolism
  • autophagy
  • BAT
  • brown adipose tissue
  • embryonic development
  • glycogen
  • glycophagy
  • lipid
  • lipid droplet biogenesis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Mayeuf-Louchart, A., Lancel, S., Sebti, Y., Pourcet, B., Loyens, A., Delhaye, S., ... Duez, H. (2019). Glycogen Dynamics Drives Lipid Droplet Biogenesis during Brown Adipocyte Differentiation. Cell Reports, 29(6), 1410-1418.e6. https://doi.org/10.1016/j.celrep.2019.09.073

Glycogen Dynamics Drives Lipid Droplet Biogenesis during Brown Adipocyte Differentiation. / Mayeuf-Louchart, Alicia; Lancel, Steve; Sebti, Yasmine; Pourcet, Benoit; Loyens, Anne; Delhaye, Stéphane; Duhem, Christian; Beauchamp, Justine; Ferri, Lise; Thorel, Quentin; Boulinguiez, Alexis; Zecchin, Mathilde; Dubois-Chevalier, Julie; Eeckhoute, Jérôme; Vaughn, Logan T.; Roach, Peter J.; Dani, Christian; Pederson, Bartholomew A.; Vincent, Stéphane D.; Staels, Bart; Duez, Hélène.

In: Cell Reports, Vol. 29, No. 6, 05.11.2019, p. 1410-1418.e6.

Research output: Contribution to journalArticle

Mayeuf-Louchart, A, Lancel, S, Sebti, Y, Pourcet, B, Loyens, A, Delhaye, S, Duhem, C, Beauchamp, J, Ferri, L, Thorel, Q, Boulinguiez, A, Zecchin, M, Dubois-Chevalier, J, Eeckhoute, J, Vaughn, LT, Roach, PJ, Dani, C, Pederson, BA, Vincent, SD, Staels, B & Duez, H 2019, 'Glycogen Dynamics Drives Lipid Droplet Biogenesis during Brown Adipocyte Differentiation', Cell Reports, vol. 29, no. 6, pp. 1410-1418.e6. https://doi.org/10.1016/j.celrep.2019.09.073
Mayeuf-Louchart A, Lancel S, Sebti Y, Pourcet B, Loyens A, Delhaye S et al. Glycogen Dynamics Drives Lipid Droplet Biogenesis during Brown Adipocyte Differentiation. Cell Reports. 2019 Nov 5;29(6):1410-1418.e6. https://doi.org/10.1016/j.celrep.2019.09.073
Mayeuf-Louchart, Alicia ; Lancel, Steve ; Sebti, Yasmine ; Pourcet, Benoit ; Loyens, Anne ; Delhaye, Stéphane ; Duhem, Christian ; Beauchamp, Justine ; Ferri, Lise ; Thorel, Quentin ; Boulinguiez, Alexis ; Zecchin, Mathilde ; Dubois-Chevalier, Julie ; Eeckhoute, Jérôme ; Vaughn, Logan T. ; Roach, Peter J. ; Dani, Christian ; Pederson, Bartholomew A. ; Vincent, Stéphane D. ; Staels, Bart ; Duez, Hélène. / Glycogen Dynamics Drives Lipid Droplet Biogenesis during Brown Adipocyte Differentiation. In: Cell Reports. 2019 ; Vol. 29, No. 6. pp. 1410-1418.e6.
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