Glycogen synthesis in the absence of glycogenin in the yeast Saccharomyces cerevisiae

María Jesús Torija, Maite Novo, Anne Lemassu, Wayne Wilson, Peter Roach, Jean François, Jean Luc Parrou

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In eukaryotic cells, glycogenin is a self-glucosylating protein that primes glycogen synthesis. In yeast, the loss of function of GLG1 and GLG2, which encode glycogenin, normally leads to the inability of cells to synthesize glycogen. In this report, we show that a small fraction of colonies from glg1glg2 mutants can switch on glycogen synthesis to levels comparable to wild-type strain. The occurrence of glycogen positive glg1glg2 colonies is strongly enhanced by the presence of a hyperactive glycogen synthase and increased even more upon deletion of TPS1. In all cases, this phenotype is reversible, indicating the stochastic nature of this synthesis, which is furthermore illustrated by colour-sectoring of colonies upon iodine-staining. Altogether, these data suggest that glycogen synthesis in the absence of glycogenin relies on a combination of several factors, including an activated glycogen synthase and as yet unknown alternative primers whose synthesis and/or distribution may be controlled by TPS1 or under epigenetic silencing.

Original languageEnglish
Pages (from-to)3999-4004
Number of pages6
JournalFEBS Letters
Volume579
Issue number18
DOIs
StatePublished - Jul 18 2005

Fingerprint

Glycogen
Yeast
Saccharomyces cerevisiae
Yeasts
Glycogen Synthase
Eukaryotic Cells
Epigenomics
Iodine
Color
Switches
glycogenin
Staining and Labeling
Phenotype
Proteins

Keywords

  • Glycogen
  • Glycogen synthase
  • Glycogenin
  • GSY2
  • TPS1
  • Trehalose

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Torija, M. J., Novo, M., Lemassu, A., Wilson, W., Roach, P., François, J., & Parrou, J. L. (2005). Glycogen synthesis in the absence of glycogenin in the yeast Saccharomyces cerevisiae. FEBS Letters, 579(18), 3999-4004. https://doi.org/10.1016/j.febslet.2005.06.007

Glycogen synthesis in the absence of glycogenin in the yeast Saccharomyces cerevisiae. / Torija, María Jesús; Novo, Maite; Lemassu, Anne; Wilson, Wayne; Roach, Peter; François, Jean; Parrou, Jean Luc.

In: FEBS Letters, Vol. 579, No. 18, 18.07.2005, p. 3999-4004.

Research output: Contribution to journalArticle

Torija, MJ, Novo, M, Lemassu, A, Wilson, W, Roach, P, François, J & Parrou, JL 2005, 'Glycogen synthesis in the absence of glycogenin in the yeast Saccharomyces cerevisiae', FEBS Letters, vol. 579, no. 18, pp. 3999-4004. https://doi.org/10.1016/j.febslet.2005.06.007
Torija, María Jesús ; Novo, Maite ; Lemassu, Anne ; Wilson, Wayne ; Roach, Peter ; François, Jean ; Parrou, Jean Luc. / Glycogen synthesis in the absence of glycogenin in the yeast Saccharomyces cerevisiae. In: FEBS Letters. 2005 ; Vol. 579, No. 18. pp. 3999-4004.
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AB - In eukaryotic cells, glycogenin is a self-glucosylating protein that primes glycogen synthesis. In yeast, the loss of function of GLG1 and GLG2, which encode glycogenin, normally leads to the inability of cells to synthesize glycogen. In this report, we show that a small fraction of colonies from glg1glg2 mutants can switch on glycogen synthesis to levels comparable to wild-type strain. The occurrence of glycogen positive glg1glg2 colonies is strongly enhanced by the presence of a hyperactive glycogen synthase and increased even more upon deletion of TPS1. In all cases, this phenotype is reversible, indicating the stochastic nature of this synthesis, which is furthermore illustrated by colour-sectoring of colonies upon iodine-staining. Altogether, these data suggest that glycogen synthesis in the absence of glycogenin relies on a combination of several factors, including an activated glycogen synthase and as yet unknown alternative primers whose synthesis and/or distribution may be controlled by TPS1 or under epigenetic silencing.

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