Glycogenin-2, a novel self-glucosylating protein involved in liver glycogen biosynthesis

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Abstract

Glycogenin is a self-glucosylating protein involved in the initiation phase of glycogen biosynthesis. A single mammalian gene had been reported to account for glycogen biogenesis in liver and muscle, the two major repositories of glycogen. We describe the characterization of novel forms of glycogenin, designated glycogenin-2 (GN-2), encoded by a second gene that is expressed preferentially in certain tissues, including liver, heart, and pancreas. Cloning of cDNAs encoding glycogenin-2 indicated the existence of multiple species, including three liver forms (GN-2α, GN-2β, and GN-2γ) generated in part by alternative splicing. Overall, GN-2 has 40-45% identity to muscle glycogenin but is 72% identical over a 200-residue segment thought to contain the catalytic domain. GN-2 expressed in Escherichia coli or COS cells is active in self-glucosylation assays, and self-glucosylated GN-2 can be elongated by skeletal muscle glycogen synthase. Antibodies raised against GN-2 produced in E. coli recognized proteins of M(r) ~66,000 present in extracts of rat liver and in cultured H4IIEC3 hepatoma cells. In H4IIEC3 cells, most of the GN-2 was present as a free protein but some was covalently associated with glycogen fractions and was only released by treatment with α-amylase. H4IIEC3 cells also expressed the muscle form of glycogenin (glycogenin-1), which was attached to a chromatographically separable glycogen fraction.

Original languageEnglish
Pages (from-to)27589-27597
Number of pages9
JournalJournal of Biological Chemistry
Volume272
Issue number44
DOIs
StatePublished - Oct 31 1997

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Liver Glycogen
Biosynthesis
Proteins
Glycogen
Liver
Muscle
glycogenin
Muscles
Escherichia coli
Genes
Liver Extracts
Glycogen Synthase
Cloning
Escherichia coli Proteins
COS Cells
Alternative Splicing
Amylases

ASJC Scopus subject areas

  • Biochemistry

Cite this

Glycogenin-2, a novel self-glucosylating protein involved in liver glycogen biosynthesis. / Mu, James; Skurat, Alexander; Roach, Peter.

In: Journal of Biological Chemistry, Vol. 272, No. 44, 31.10.1997, p. 27589-27597.

Research output: Contribution to journalArticle

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