Glycomics analyses of tear fluid for the diagnostic detection of ocular rosacea

Hyun Joo An, Milady Ninonuevo, Jennifer Aguilan, Hao Liu, Carlito B. Lebrilla, Lenio S. Alvarenga, Mark J. Mannis

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A Glycomics approach to detect disease is illustrated in the analyses of human tear fluid for rosacea. The diagnosis of ocular rosacea is particularly challenging in a subgroup of patients that do not present with typical facial skin findings but have ocular signs and symptoms. Indeed, up to 90% of patients with ocular rosacea may have neither obvious roseatic skin changes nor a previous diagnosis of rosacea. Tear fluid was collected from 37 subjects (21 controls and 16 patients with ocular rosacea) after conjunctival stimulation with filter (Schirmer) paper. O-linked oligosaccharides were released from tear fluid by β-elimination and then purified using solid-phase extraction. Mass spectra were recorded on an external source HiResMALDI with a 7.0 T magnet. Mass spectra were obtained in both the positive and negative modes. However, signals were stronger in the negative mode. Tear fluid samples from rosacea patients yielded distinctive clusters of peaks that extend to higher masses. Patients with rosacea presented several oligomeric series that were not found in the controls. To discriminate the ocular rosacea cases from the normal controls, the sum of absolute intensities of 13 series corresponding to nearly 50 identified mass spectrum peaks was used. Thirty-six out of the 37 samples were correctly classified. This yields a sensitivity of 100% (95% CI 79.5-100) and specificity of 95.2% (95% CI 76.2-99.9). The high abundance of oligosaccharides in the tear fluid of patients with rosacea may lead to an objective diagnostic marker for the disease.

Original languageEnglish (US)
Pages (from-to)1981-1987
Number of pages7
JournalJournal of Proteome Research
Volume4
Issue number6
DOIs
StatePublished - Nov 1 2005
Externally publishedYes

Fingerprint

Glycomics
Rosacea
Tears
Fluids
Oligosaccharides
Skin
Magnets
Solid Phase Extraction
Signs and Symptoms

Keywords

  • Biomarker
  • Glycomics
  • MALDI-FTMS
  • Mucin
  • O-linked oligosaccharides
  • Ocular rosacea

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

An, H. J., Ninonuevo, M., Aguilan, J., Liu, H., Lebrilla, C. B., Alvarenga, L. S., & Mannis, M. J. (2005). Glycomics analyses of tear fluid for the diagnostic detection of ocular rosacea. Journal of Proteome Research, 4(6), 1981-1987. https://doi.org/10.1021/pr0501620

Glycomics analyses of tear fluid for the diagnostic detection of ocular rosacea. / An, Hyun Joo; Ninonuevo, Milady; Aguilan, Jennifer; Liu, Hao; Lebrilla, Carlito B.; Alvarenga, Lenio S.; Mannis, Mark J.

In: Journal of Proteome Research, Vol. 4, No. 6, 01.11.2005, p. 1981-1987.

Research output: Contribution to journalArticle

An, HJ, Ninonuevo, M, Aguilan, J, Liu, H, Lebrilla, CB, Alvarenga, LS & Mannis, MJ 2005, 'Glycomics analyses of tear fluid for the diagnostic detection of ocular rosacea', Journal of Proteome Research, vol. 4, no. 6, pp. 1981-1987. https://doi.org/10.1021/pr0501620
An, Hyun Joo ; Ninonuevo, Milady ; Aguilan, Jennifer ; Liu, Hao ; Lebrilla, Carlito B. ; Alvarenga, Lenio S. ; Mannis, Mark J. / Glycomics analyses of tear fluid for the diagnostic detection of ocular rosacea. In: Journal of Proteome Research. 2005 ; Vol. 4, No. 6. pp. 1981-1987.
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