Glycoprotein C of herpes simplex virus type 1 plays a principal role in the adsorption of virus to cells and in infectivity

Betsy C. Herold, Darrell WuDunn, Manette Soltys, Patricia G. Spear

Research output: Contribution to journalArticle

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Abstract

The purpose of this study was to identify the herpes simplex virus glycopretein(s) that mediates the adsorption of virions to cells. Because heparan sulfate moieties of cell surface preteogiycaas serve as the receptors for herpes simplex virus adsorption, we tested whether any of the viral glycopreteins could bind to heparin-Sepharesc in affinity chromatography experiments. Two glycopreteins, gB and gC, bound to heparin-Sepharose and could be eluted with soluble heparin. In order to determine whether virions devoid of gC or gB were impaired for adsorption, we quantitated the binding of wild-type and mutant virions to cells. We found that at equivalent input concentrations of purified virions, significantly fewer gC-negative virions bound to cells than did wild-type or gB-negative virions. In addition, the gC-negative virions that bound to cells showed a significant delay in penetration compared with wild-type virus. The impairments in adsorption and penetration of the gC-negative virions can account for their reduced PFU/particle ratios, which were found to be about 5 to 10% that of wild-type virions, depending on the host cell. Although gC is dispensable for replication of herpes simplex virus in cell culture, it clearly facilitates virion adsorption and enhances infectivity by about a factor of 10.

Original languageEnglish (US)
Pages (from-to)1090-1098
Number of pages9
JournalJournal of Virology
Volume65
Issue number3
StatePublished - 1991
Externally publishedYes

Fingerprint

Human herpesvirus 1
Human Herpesvirus 1
virion
Virion
Adsorption
glycoproteins
adsorption
pathogenicity
Viruses
viruses
herpes simplex
cells
heparin
Simplexvirus
Heparin
glycoprotein gC, herpes simplex virus type 1
Heparitin Sulfate
affinity chromatography
Affinity Chromatography
agarose

ASJC Scopus subject areas

  • Immunology

Cite this

Glycoprotein C of herpes simplex virus type 1 plays a principal role in the adsorption of virus to cells and in infectivity. / Herold, Betsy C.; WuDunn, Darrell; Soltys, Manette; Spear, Patricia G.

In: Journal of Virology, Vol. 65, No. 3, 1991, p. 1090-1098.

Research output: Contribution to journalArticle

Herold, Betsy C. ; WuDunn, Darrell ; Soltys, Manette ; Spear, Patricia G. / Glycoprotein C of herpes simplex virus type 1 plays a principal role in the adsorption of virus to cells and in infectivity. In: Journal of Virology. 1991 ; Vol. 65, No. 3. pp. 1090-1098.
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