Glycosylation requirements for intracellular transport and function of the hemagglutinin of influenza virus

Patricia Gallagher, Jean M. Henneberry, Joseph F. Sambrook, Mary Jane H Gething

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The contribution of each of the seven asparagine-linked oligosaccharide side chains on the hemagglutinin of the A/Aichi/68 (X31) strain of influenza virus was assessed with respect to its effect on the folding, intracellular transport, and biological activities of the molecule. Twenty mutant influenza virus hemagglutinins were constructed and expressed, each of which had one or more of the seven glycosylation sites removed. Investigations of these mutant hemagglutinins indicated that (i) no individual oligosaccharide side chain is necessary or sufficient for the folding, intracellular transport, or function of the molecule, (ii) at least five oligosaccharide side chains are required for the X31 hemagglutinin molecule to move along the exocytic pathway to the plasma membrane, and (iii) mutant hemagglutinins having less than five oligosaccharide side chains form intracellular aggregates and are retained in the endoplasmic reticulum.

Original languageEnglish (US)
Pages (from-to)7136-7145
Number of pages10
JournalJournal of Virology
Volume66
Issue number12
StatePublished - Dec 1992
Externally publishedYes

Fingerprint

Hemagglutinins
hemagglutinins
Orthomyxoviridae
glycosylation
Glycosylation
Oligosaccharides
oligosaccharides
mutants
Biological Transport
Asparagine
protein aggregates
asparagine
Endoplasmic Reticulum
endoplasmic reticulum
bioactive properties
plasma membrane
Cell Membrane

ASJC Scopus subject areas

  • Immunology

Cite this

Gallagher, P., Henneberry, J. M., Sambrook, J. F., & Gething, M. J. H. (1992). Glycosylation requirements for intracellular transport and function of the hemagglutinin of influenza virus. Journal of Virology, 66(12), 7136-7145.

Glycosylation requirements for intracellular transport and function of the hemagglutinin of influenza virus. / Gallagher, Patricia; Henneberry, Jean M.; Sambrook, Joseph F.; Gething, Mary Jane H.

In: Journal of Virology, Vol. 66, No. 12, 12.1992, p. 7136-7145.

Research output: Contribution to journalArticle

Gallagher, P, Henneberry, JM, Sambrook, JF & Gething, MJH 1992, 'Glycosylation requirements for intracellular transport and function of the hemagglutinin of influenza virus', Journal of Virology, vol. 66, no. 12, pp. 7136-7145.
Gallagher P, Henneberry JM, Sambrook JF, Gething MJH. Glycosylation requirements for intracellular transport and function of the hemagglutinin of influenza virus. Journal of Virology. 1992 Dec;66(12):7136-7145.
Gallagher, Patricia ; Henneberry, Jean M. ; Sambrook, Joseph F. ; Gething, Mary Jane H. / Glycosylation requirements for intracellular transport and function of the hemagglutinin of influenza virus. In: Journal of Virology. 1992 ; Vol. 66, No. 12. pp. 7136-7145.
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