Glypican-3 expression is markedly decreased in human gastric cancer but not in esophageal cancer

Zhaowen Zhu, Helmut Friess, Jörg Kleeff, Li Wang, Martin Wirtz, Arthur Zimmermann, Murray Korc, Markus W. Büchler

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: Deregulation of the expression of glypican-3, a heparan sulfate proteoglycan, has been demonstrated in several human cancers. Methods: In the present study, glypican-3 mRNA expression was analyzed by Northern blotting and in situ hybridization in 20 normal and 41 cancerous esophageal specimens as well as in 15 normal and 32 cancerous gastric tissues. Results: Glypican-3 mRNA was expressed in both normal and esophageal cancer tissues without a significant difference between normal and cancerous tissues, and without a correlation with histological type, tumor stage, tumor grade, or patient survival. Moderate to strong glypican-3 mRNA signals were found in the cytoplasm of squamous epithelial cells of the normal esophagus. In both squamous and adenocarcinomas of the esophagus glypican-3 mRNA signals were also moderately to strongly present in the cytoplasm of the cancer cells. In gastric tissues, glypican-3 mRNA was present in 53% of normal gastric tissue samples, but was below the detection level in all examined gastric cancer samples. Glypican-3 mRNA signals were moderately to strongly present in the cytoplasm of gastric mucosal epithelial cells, but were only very faintly present in some cancer cells. Conclusions: Glypican-3 may be involved in the growth control of normal esophageal and gastric epithelial cells. Furthermore, our results suggest that glypican-3 may play a tumor suppressor role in gastric but not in esophageal cancer.

Original languageEnglish (US)
Pages (from-to)78-83
Number of pages6
JournalAmerican Journal of Surgery
Volume184
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Glypicans
Esophageal Neoplasms
Stomach Neoplasms
Stomach
Messenger RNA
Neoplasms
Cytoplasm
Epithelial Cells
Heparan Sulfate Proteoglycans
Northern Blotting
Esophagus
In Situ Hybridization

Keywords

  • Esophageal cancer
  • Gastric cancer
  • Glypican-3
  • Heparan sulfate proteoglycan

ASJC Scopus subject areas

  • Surgery

Cite this

Zhu, Z., Friess, H., Kleeff, J., Wang, L., Wirtz, M., Zimmermann, A., ... Büchler, M. W. (2002). Glypican-3 expression is markedly decreased in human gastric cancer but not in esophageal cancer. American Journal of Surgery, 184(1), 78-83. https://doi.org/10.1016/S0002-9610(02)00884-X

Glypican-3 expression is markedly decreased in human gastric cancer but not in esophageal cancer. / Zhu, Zhaowen; Friess, Helmut; Kleeff, Jörg; Wang, Li; Wirtz, Martin; Zimmermann, Arthur; Korc, Murray; Büchler, Markus W.

In: American Journal of Surgery, Vol. 184, No. 1, 2002, p. 78-83.

Research output: Contribution to journalArticle

Zhu, Z, Friess, H, Kleeff, J, Wang, L, Wirtz, M, Zimmermann, A, Korc, M & Büchler, MW 2002, 'Glypican-3 expression is markedly decreased in human gastric cancer but not in esophageal cancer', American Journal of Surgery, vol. 184, no. 1, pp. 78-83. https://doi.org/10.1016/S0002-9610(02)00884-X
Zhu, Zhaowen ; Friess, Helmut ; Kleeff, Jörg ; Wang, Li ; Wirtz, Martin ; Zimmermann, Arthur ; Korc, Murray ; Büchler, Markus W. / Glypican-3 expression is markedly decreased in human gastric cancer but not in esophageal cancer. In: American Journal of Surgery. 2002 ; Vol. 184, No. 1. pp. 78-83.
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AU - Zhu, Zhaowen

AU - Friess, Helmut

AU - Kleeff, Jörg

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AU - Wirtz, Martin

AU - Zimmermann, Arthur

AU - Korc, Murray

AU - Büchler, Markus W.

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AB - Background: Deregulation of the expression of glypican-3, a heparan sulfate proteoglycan, has been demonstrated in several human cancers. Methods: In the present study, glypican-3 mRNA expression was analyzed by Northern blotting and in situ hybridization in 20 normal and 41 cancerous esophageal specimens as well as in 15 normal and 32 cancerous gastric tissues. Results: Glypican-3 mRNA was expressed in both normal and esophageal cancer tissues without a significant difference between normal and cancerous tissues, and without a correlation with histological type, tumor stage, tumor grade, or patient survival. Moderate to strong glypican-3 mRNA signals were found in the cytoplasm of squamous epithelial cells of the normal esophagus. In both squamous and adenocarcinomas of the esophagus glypican-3 mRNA signals were also moderately to strongly present in the cytoplasm of the cancer cells. In gastric tissues, glypican-3 mRNA was present in 53% of normal gastric tissue samples, but was below the detection level in all examined gastric cancer samples. Glypican-3 mRNA signals were moderately to strongly present in the cytoplasm of gastric mucosal epithelial cells, but were only very faintly present in some cancer cells. Conclusions: Glypican-3 may be involved in the growth control of normal esophageal and gastric epithelial cells. Furthermore, our results suggest that glypican-3 may play a tumor suppressor role in gastric but not in esophageal cancer.

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