GMS is superior to PAS for diagnosis of onychomycosis

Zandra D'Hue, Susan Perkins, Steven D. Billings

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: Onychomycosis is a common cause of deformed nails. Periodic acid-Schiff (PAS) stains are more sensitive than fungal cultures for diagnosing onychomycosis. We performed a retrospective study comparing the use of PAS and Gomori methenamine silver (GMS) stains for histopathologic diagnosis of onychomycosis. Methods: GMS stains were performed on 20 PAS-positive and 51 PAS-negative cases from nail biopsies with a clinical diagnosis of onychomycosis. The PAS stained slides and GMS stained slides were evaluated for the presence offungal hyphae. The results were analyzed with McNemar's test. Results: All 20 PAS-positive cases were also positive with GMS stains. Of the 51 PAS-negative cases, GMS stains detected an additional five cases with fungal hyphae. GMS stains were quantitatively superior (p < 0.0253). GMS stains were also qualitatively superior. More fungal hyphae were highlighted and fungal hyphae were more easily recognized on low or medium power magnification. Conclusions: GMS stains are superior to PAS stains for the routine diagnosis of onychomycosis.

Original languageEnglish
Pages (from-to)745-747
Number of pages3
JournalJournal of Cutaneous Pathology
Volume35
Issue number8
DOIs
StatePublished - Aug 2008

Fingerprint

Methenamine
Onychomycosis
Periodic Acid
Coloring Agents
Hyphae
Nails
Retrospective Studies
Biopsy

ASJC Scopus subject areas

  • Dermatology
  • Pathology and Forensic Medicine
  • Histology

Cite this

GMS is superior to PAS for diagnosis of onychomycosis. / D'Hue, Zandra; Perkins, Susan; Billings, Steven D.

In: Journal of Cutaneous Pathology, Vol. 35, No. 8, 08.2008, p. 745-747.

Research output: Contribution to journalArticle

D'Hue, Zandra ; Perkins, Susan ; Billings, Steven D. / GMS is superior to PAS for diagnosis of onychomycosis. In: Journal of Cutaneous Pathology. 2008 ; Vol. 35, No. 8. pp. 745-747.
@article{bf9ad59154de4fee8b7e5b551cb022c8,
title = "GMS is superior to PAS for diagnosis of onychomycosis",
abstract = "Background: Onychomycosis is a common cause of deformed nails. Periodic acid-Schiff (PAS) stains are more sensitive than fungal cultures for diagnosing onychomycosis. We performed a retrospective study comparing the use of PAS and Gomori methenamine silver (GMS) stains for histopathologic diagnosis of onychomycosis. Methods: GMS stains were performed on 20 PAS-positive and 51 PAS-negative cases from nail biopsies with a clinical diagnosis of onychomycosis. The PAS stained slides and GMS stained slides were evaluated for the presence offungal hyphae. The results were analyzed with McNemar's test. Results: All 20 PAS-positive cases were also positive with GMS stains. Of the 51 PAS-negative cases, GMS stains detected an additional five cases with fungal hyphae. GMS stains were quantitatively superior (p < 0.0253). GMS stains were also qualitatively superior. More fungal hyphae were highlighted and fungal hyphae were more easily recognized on low or medium power magnification. Conclusions: GMS stains are superior to PAS stains for the routine diagnosis of onychomycosis.",
author = "Zandra D'Hue and Susan Perkins and Billings, {Steven D.}",
year = "2008",
month = "8",
doi = "10.1111/j.1600-0560.2007.00890.x",
language = "English",
volume = "35",
pages = "745--747",
journal = "Journal of Cutaneous Pathology",
issn = "0303-6987",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - GMS is superior to PAS for diagnosis of onychomycosis

AU - D'Hue, Zandra

AU - Perkins, Susan

AU - Billings, Steven D.

PY - 2008/8

Y1 - 2008/8

N2 - Background: Onychomycosis is a common cause of deformed nails. Periodic acid-Schiff (PAS) stains are more sensitive than fungal cultures for diagnosing onychomycosis. We performed a retrospective study comparing the use of PAS and Gomori methenamine silver (GMS) stains for histopathologic diagnosis of onychomycosis. Methods: GMS stains were performed on 20 PAS-positive and 51 PAS-negative cases from nail biopsies with a clinical diagnosis of onychomycosis. The PAS stained slides and GMS stained slides were evaluated for the presence offungal hyphae. The results were analyzed with McNemar's test. Results: All 20 PAS-positive cases were also positive with GMS stains. Of the 51 PAS-negative cases, GMS stains detected an additional five cases with fungal hyphae. GMS stains were quantitatively superior (p < 0.0253). GMS stains were also qualitatively superior. More fungal hyphae were highlighted and fungal hyphae were more easily recognized on low or medium power magnification. Conclusions: GMS stains are superior to PAS stains for the routine diagnosis of onychomycosis.

AB - Background: Onychomycosis is a common cause of deformed nails. Periodic acid-Schiff (PAS) stains are more sensitive than fungal cultures for diagnosing onychomycosis. We performed a retrospective study comparing the use of PAS and Gomori methenamine silver (GMS) stains for histopathologic diagnosis of onychomycosis. Methods: GMS stains were performed on 20 PAS-positive and 51 PAS-negative cases from nail biopsies with a clinical diagnosis of onychomycosis. The PAS stained slides and GMS stained slides were evaluated for the presence offungal hyphae. The results were analyzed with McNemar's test. Results: All 20 PAS-positive cases were also positive with GMS stains. Of the 51 PAS-negative cases, GMS stains detected an additional five cases with fungal hyphae. GMS stains were quantitatively superior (p < 0.0253). GMS stains were also qualitatively superior. More fungal hyphae were highlighted and fungal hyphae were more easily recognized on low or medium power magnification. Conclusions: GMS stains are superior to PAS stains for the routine diagnosis of onychomycosis.

UR - http://www.scopus.com/inward/record.url?scp=58149198848&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149198848&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0560.2007.00890.x

DO - 10.1111/j.1600-0560.2007.00890.x

M3 - Article

C2 - 18331569

AN - SCOPUS:58149198848

VL - 35

SP - 745

EP - 747

JO - Journal of Cutaneous Pathology

JF - Journal of Cutaneous Pathology

SN - 0303-6987

IS - 8

ER -