Golgi galactosyltransferase contains serine-linked phosphate.

G. J. Strous, P. van Kerkhof, Robert Fallon, A. L. Schwartz

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In HeLa and HepG2 cells the Golgi complex enzyme galactosyltransferase became phosphorylated following incubation with 32Pi-Analysis on sodium dodecyl sulphate/polyacrylamide gel electrophoresis revealed incorporation of 32P into the mature 54-kDa form. This phosphorylation was independent of protein synthesis. Serine was identified as the sole phosphorylated amino acid; no radioactive phosphate was detected on N-linked oligosaccharide. The phosphate-labelled galactosyltransferase has the same turnover as [35S]methionine-labelled polypeptides (t1/2 = 20 h). Soluble enzyme, released by the cells, contained very little phosphate relative to that which remained cell-associated. Charge heterogeneity arising from phosphorylation contributes in part to the heterodispersed appearance of the enzyme on two-dimensional gels, as the degree of radioactive phosphate differs among the different iso-enzymes.

Original languageEnglish (US)
Pages (from-to)307-311
Number of pages5
JournalEuropean Journal of Biochemistry
Volume169
Issue number2
StatePublished - Dec 1 1987
Externally publishedYes

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Galactosyltransferases
Phosphoserine
Serine
Phosphates
Phosphorylation
Enzymes
Hep G2 Cells
Golgi Apparatus
Electrophoresis
Oligosaccharides
HeLa Cells
Sodium Dodecyl Sulfate
Methionine
Polyacrylamide Gel Electrophoresis
Gels
Amino Acids
Peptides
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Strous, G. J., van Kerkhof, P., Fallon, R., & Schwartz, A. L. (1987). Golgi galactosyltransferase contains serine-linked phosphate. European Journal of Biochemistry, 169(2), 307-311.

Golgi galactosyltransferase contains serine-linked phosphate. / Strous, G. J.; van Kerkhof, P.; Fallon, Robert; Schwartz, A. L.

In: European Journal of Biochemistry, Vol. 169, No. 2, 01.12.1987, p. 307-311.

Research output: Contribution to journalArticle

Strous, GJ, van Kerkhof, P, Fallon, R & Schwartz, AL 1987, 'Golgi galactosyltransferase contains serine-linked phosphate.', European Journal of Biochemistry, vol. 169, no. 2, pp. 307-311.
Strous GJ, van Kerkhof P, Fallon R, Schwartz AL. Golgi galactosyltransferase contains serine-linked phosphate. European Journal of Biochemistry. 1987 Dec 1;169(2):307-311.
Strous, G. J. ; van Kerkhof, P. ; Fallon, Robert ; Schwartz, A. L. / Golgi galactosyltransferase contains serine-linked phosphate. In: European Journal of Biochemistry. 1987 ; Vol. 169, No. 2. pp. 307-311.
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