Granulocyte/macrophage colony-stimulating factor and accessory cells modulate radioprotection by purified hematopoietic cells

Tamiko R. Katsumoto, Jennifer Duda, Andrew Kim, Zabihullah Wardak, Glenn Dranoff, D. Wade Clapp, Kevin Shannon

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Granulocyte/macrophage colony-stimulating factor (GM-CSF) promotes the survival, proliferation, and differentiation of myeloid lineage cells and regulates chemotaxis and adhesion. However, mice in which the genes encoding GM-CSF (Gmcsf) or the β common subunit of the GM-CSF receptor (βc) are inactivated display normal steady-state hematopoiesis. Here, we show that host GM-CSF signaling strongly modulates the ability of donor hematopoietic cells to radioprotect lethally irradiated mice. Although bone marrow mononuclear cells efficiently rescue Gmcsf mutant recipients, fetal liver cells and Sca1 + lin-/dim marrow cells are markedly impaired. This defect is partially attributable to accessory cells that are more prevalent in bone marrow. In contrast, Gmcsf-deficient hematopoietic stem cells demonstrate normal proliferative potentials. Short-term survival is also impaired in irradiated βc mutant recipients transplanted with fetal liver or bone marrow. These data demonstrate a nonredundant function of GM-CSF in radioprotection by donor hematopoietic cells that may prove relevant in clinical transplantation.

Original languageEnglish (US)
Pages (from-to)853-858
Number of pages6
JournalJournal of Experimental Medicine
Volume201
Issue number6
DOIs
StatePublished - Mar 21 2005

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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