Granulocyte/macrophage colony-stimulating factor and accessory cells modulate radioprotection by purified hematopoietic cells

Tamiko R. Katsumoto, Jennifer Duda, Andrew Kim, Zabihullah Wardak, Glenn Dranoff, D. Clapp, Kevin Shannon

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Granulocyte/macrophage colony-stimulating factor (GM-CSF) promotes the survival, proliferation, and differentiation of myeloid lineage cells and regulates chemotaxis and adhesion. However, mice in which the genes encoding GM-CSF (Gmcsf) or the β common subunit of the GM-CSF receptor (βc) are inactivated display normal steady-state hematopoiesis. Here, we show that host GM-CSF signaling strongly modulates the ability of donor hematopoietic cells to radioprotect lethally irradiated mice. Although bone marrow mononuclear cells efficiently rescue Gmcsf mutant recipients, fetal liver cells and Sca1 + lin-/dim marrow cells are markedly impaired. This defect is partially attributable to accessory cells that are more prevalent in bone marrow. In contrast, Gmcsf-deficient hematopoietic stem cells demonstrate normal proliferative potentials. Short-term survival is also impaired in irradiated βc mutant recipients transplanted with fetal liver or bone marrow. These data demonstrate a nonredundant function of GM-CSF in radioprotection by donor hematopoietic cells that may prove relevant in clinical transplantation.

Original languageEnglish
Pages (from-to)853-858
Number of pages6
JournalJournal of Experimental Medicine
Volume201
Issue number6
DOIs
StatePublished - Mar 21 2005

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Granulocyte-Macrophage Colony-Stimulating Factor
Bone Marrow
Granulocyte-Macrophage Colony-Stimulating Factor Receptors
Aptitude
Liver
Hematopoiesis
Chemotaxis
Myeloid Cells
Hematopoietic Stem Cells
Bone Marrow Cells
Transplantation
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Granulocyte/macrophage colony-stimulating factor and accessory cells modulate radioprotection by purified hematopoietic cells. / Katsumoto, Tamiko R.; Duda, Jennifer; Kim, Andrew; Wardak, Zabihullah; Dranoff, Glenn; Clapp, D.; Shannon, Kevin.

In: Journal of Experimental Medicine, Vol. 201, No. 6, 21.03.2005, p. 853-858.

Research output: Contribution to journalArticle

Katsumoto, Tamiko R. ; Duda, Jennifer ; Kim, Andrew ; Wardak, Zabihullah ; Dranoff, Glenn ; Clapp, D. ; Shannon, Kevin. / Granulocyte/macrophage colony-stimulating factor and accessory cells modulate radioprotection by purified hematopoietic cells. In: Journal of Experimental Medicine. 2005 ; Vol. 201, No. 6. pp. 853-858.
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