Grap negatively regulates T-cell receptor-elicited lymphocyte proliferation and interleukin-2 induction

Randy Shen, Ying Bin Ouyang, Cheng Kui Qu, Andres Alonso, Lindsey Sperzel, Tomas Mustelin, Mark H. Kaplan, Gen Sheng Feng

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Grb-2-related adaptor protein (Grap) is a Grb2-like SH3-SH2-SH3 adaptor protein with expression restricted to lymphoid tissues. Grap-/- lymphocytes isolated from targeted Grap-deficient mice exhibited enhanced proliferation, interleukin-2 production, and c-fos induction in response to mitogenic T-cell receptor (TCR) stimulation, compared to wild-type cells. Ectopic expression of Grap led to a suppression of Elk-1-directed transcription induced by the Ras/Erk pathway, without having effects on gene expression mediated by Jnk and p38 mitogen-activated protein kinases. Together, these data suggest that Grap, unlike Grb2, acts as a negative regulator of TCR-stimulated intracellular signaling by downregulating signal relay through the Ras/Erk pathway.

Original languageEnglish (US)
Pages (from-to)3230-3236
Number of pages7
JournalMolecular and cellular biology
Volume22
Issue number10
DOIs
StatePublished - May 9 2002
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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