Grap negatively regulates T-cell receptor-elicited lymphocyte proliferation and interleukin-2 induction

Randy Shen, Ying Bin Ouyang, Cheng Kui Qu, Andres Alonso, Lindsey Sperzel, Tomas Mustelin, Mark H. Kaplan, Gen Sheng Feng

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Grb-2-related adaptor protein (Grap) is a Grb2-like SH3-SH2-SH3 adaptor protein with expression restricted to lymphoid tissues. Grap-/- lymphocytes isolated from targeted Grap-deficient mice exhibited enhanced proliferation, interleukin-2 production, and c-fos induction in response to mitogenic T-cell receptor (TCR) stimulation, compared to wild-type cells. Ectopic expression of Grap led to a suppression of Elk-1-directed transcription induced by the Ras/Erk pathway, without having effects on gene expression mediated by Jnk and p38 mitogen-activated protein kinases. Together, these data suggest that Grap, unlike Grb2, acts as a negative regulator of TCR-stimulated intracellular signaling by downregulating signal relay through the Ras/Erk pathway.

Original languageEnglish (US)
Pages (from-to)3230-3236
Number of pages7
JournalMolecular and cellular biology
Volume22
Issue number10
DOIs
StatePublished - May 9 2002

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T-Cell Antigen Receptor
Interleukin-2
Lymphocytes
Proteins
Lymphoid Tissue
p38 Mitogen-Activated Protein Kinases
Down-Regulation
Gene Expression

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Shen, R., Ouyang, Y. B., Qu, C. K., Alonso, A., Sperzel, L., Mustelin, T., ... Feng, G. S. (2002). Grap negatively regulates T-cell receptor-elicited lymphocyte proliferation and interleukin-2 induction. Molecular and cellular biology, 22(10), 3230-3236. https://doi.org/10.1128/MCB.22.10.3230-3236.2002

Grap negatively regulates T-cell receptor-elicited lymphocyte proliferation and interleukin-2 induction. / Shen, Randy; Ouyang, Ying Bin; Qu, Cheng Kui; Alonso, Andres; Sperzel, Lindsey; Mustelin, Tomas; Kaplan, Mark H.; Feng, Gen Sheng.

In: Molecular and cellular biology, Vol. 22, No. 10, 09.05.2002, p. 3230-3236.

Research output: Contribution to journalArticle

Shen, Randy ; Ouyang, Ying Bin ; Qu, Cheng Kui ; Alonso, Andres ; Sperzel, Lindsey ; Mustelin, Tomas ; Kaplan, Mark H. ; Feng, Gen Sheng. / Grap negatively regulates T-cell receptor-elicited lymphocyte proliferation and interleukin-2 induction. In: Molecular and cellular biology. 2002 ; Vol. 22, No. 10. pp. 3230-3236.
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