Graphene-VP40 interactions and potential disruption of the Ebola virus matrix filaments

Jeevan B. GC, Rudramani Pokhrel, Nisha Bhattarai, Kristen A. Johnson, Bernard S. Gerstman, Robert Stahelin, Prem P. Chapagain

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Ebola virus infections cause hemorrhagic fever that often results in very high fatality rates. In addition to exploring vaccines, development of drugs is also essential for treating the disease and preventing the spread of the infection. The Ebola virus matrix protein VP40 exists in various conformational and oligomeric forms and is a potential pharmacological target for disrupting the virus life-cycle. Here we explored graphene-VP40 interactions using molecular dynamics simulations and graphene pelleting assays. We found that graphene sheets associate strongly with VP40 at various interfaces. We also found that the graphene is able to disrupt the C-terminal domain (CTD-CTD) interface of VP40 hexamers. This VP40 hexamer-hexamer interface is crucial in forming the Ebola viral matrix and disruption of this interface may provide a method to use graphene or similar nanoparticle based solutions as a disinfectant that can significantly reduce the spread of the disease and prevent an Ebola epidemic.

Original languageEnglish (US)
JournalBiochemical and Biophysical Research Communications
DOIs
StateAccepted/In press - 2017

Fingerprint

Ebolavirus
Graphite
Viruses
Ebola Hemorrhagic Fever
Pelletizing
Disinfectants
Molecular Dynamics Simulation
Life Cycle Stages
Nanoparticles
Molecular dynamics
Life cycle
Assays
Fever
Vaccines
Pharmacology
Computer simulation
Infection
Pharmaceutical Preparations
Proteins

Keywords

  • Ebola virus
  • Graphene sheet
  • Matrix filaments
  • Nanotherapy
  • VP40

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Graphene-VP40 interactions and potential disruption of the Ebola virus matrix filaments. / GC, Jeevan B.; Pokhrel, Rudramani; Bhattarai, Nisha; Johnson, Kristen A.; Gerstman, Bernard S.; Stahelin, Robert; Chapagain, Prem P.

In: Biochemical and Biophysical Research Communications, 2017.

Research output: Contribution to journalArticle

GC, Jeevan B. ; Pokhrel, Rudramani ; Bhattarai, Nisha ; Johnson, Kristen A. ; Gerstman, Bernard S. ; Stahelin, Robert ; Chapagain, Prem P. / Graphene-VP40 interactions and potential disruption of the Ebola virus matrix filaments. In: Biochemical and Biophysical Research Communications. 2017.
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