Green tea catechin inhibits the activity and neutrophil release ofMatrix Metalloproteinase-9

Wan K. Kim-Park, Eman S. Allam, Jadesada Palasuk, Michael Kowolik, Kichuel K. Park, L. Windsor

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Green tea (Camellia sinensis; [U+7DA0][U+8336] lü` chá) extracts have been shown to possess anti-oxidant and anti-inflammatory effects in various cell types. Green tea extract (GTX) has been shown to significantly inhibit the activity of collagenase-3 (matrix metalloproteinase-13 (MMP-13)) invitro. MMPs, such as MMP-9, are known to be involved in many inflammatory diseases including periodontal disease. GTX and a major catechin, epigallocatechin-gallate (EGCG), were examined for their ability to inhibit purified MMP-9 activity and its release from stimulated neutrophils.Methanol extract of Green tea and commercially purchased EGCG (>95 % purity) were tested invitro for their ability to inhibit MMP-9 activity and/or its release from neutrophils using a β-casein cleavage assay and gelatin zymography, respectively. Statistical analysis was performed by Student's t-test.GTX and EGCG at 0.1% (w/v) completely inhibited the activity of MMP-9. In addition, GTX and EGCG (0.1 %) significantly inhibited (p

Original languageEnglish (US)
JournalJournal of Traditional and Complementary Medicine
DOIs
StateAccepted/In press - Dec 17 2014

Fingerprint

Catechin
Metalloproteases
Tea
Matrix Metalloproteinases
Neutrophils
Matrix Metalloproteinase 13
Camellia sinensis
Periodontal Diseases
Gelatin
Caseins
Oxidants
Methanol
Anti-Inflammatory Agents
Students
epigallocatechin gallate

Keywords

  • Epigallocatechin-gallate
  • Green tea catechin
  • Matrix metalloproteinases
  • Neutrophils
  • Periodontal diseases

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Green tea catechin inhibits the activity and neutrophil release ofMatrix Metalloproteinase-9. / Kim-Park, Wan K.; Allam, Eman S.; Palasuk, Jadesada; Kowolik, Michael; Park, Kichuel K.; Windsor, L.

In: Journal of Traditional and Complementary Medicine, 17.12.2014.

Research output: Contribution to journalArticle

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AU - Palasuk, Jadesada

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AU - Windsor, L.

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