Growth factor production by sarcoma cells in primary culture

R. S. Bell, D. F. Bell, J. Jacob, Stephen Trippel, M. C. Gebhardt, H. J. Mankin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cell lines derived from human sarcomas have been previously shown to produce a protein growth factor which, similar to platelet-derived growth factor (PDGF), can induce competence for mitosis in fibroblasts. Whether this factor production is an important feature of sarcomas in vivo or simply an artefact caused by long-term culture conditions is unclear, however. We demonstrated growth factor activity in conditioned medium from six of 11 primary sarcoma cultures, utilizing flow cytometric analysis of DNA to monitor the presence of sarcoma cells in cultures. Cells isolated from control tissues failed to show a similar mitotic effect.

Original languageEnglish (US)
Pages (from-to)468-473
Number of pages6
JournalJournal of Orthopaedic Research
Volume7
Issue number4
StatePublished - 1989
Externally publishedYes

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Primary Cell Culture
Sarcoma
Intercellular Signaling Peptides and Proteins
Platelet-Derived Growth Factor
Conditioned Culture Medium
Mitosis
Artifacts
Mental Competency
Cell Culture Techniques
Fibroblasts
Cell Line
DNA
Proteins

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Bell, R. S., Bell, D. F., Jacob, J., Trippel, S., Gebhardt, M. C., & Mankin, H. J. (1989). Growth factor production by sarcoma cells in primary culture. Journal of Orthopaedic Research, 7(4), 468-473.

Growth factor production by sarcoma cells in primary culture. / Bell, R. S.; Bell, D. F.; Jacob, J.; Trippel, Stephen; Gebhardt, M. C.; Mankin, H. J.

In: Journal of Orthopaedic Research, Vol. 7, No. 4, 1989, p. 468-473.

Research output: Contribution to journalArticle

Bell, RS, Bell, DF, Jacob, J, Trippel, S, Gebhardt, MC & Mankin, HJ 1989, 'Growth factor production by sarcoma cells in primary culture', Journal of Orthopaedic Research, vol. 7, no. 4, pp. 468-473.
Bell RS, Bell DF, Jacob J, Trippel S, Gebhardt MC, Mankin HJ. Growth factor production by sarcoma cells in primary culture. Journal of Orthopaedic Research. 1989;7(4):468-473.
Bell, R. S. ; Bell, D. F. ; Jacob, J. ; Trippel, Stephen ; Gebhardt, M. C. ; Mankin, H. J. / Growth factor production by sarcoma cells in primary culture. In: Journal of Orthopaedic Research. 1989 ; Vol. 7, No. 4. pp. 468-473.
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