Growth factor regulation of growth factors in articular chondrocytes

Shuiliang Shi, Scott Mercer, George J. Eckert, Stephen Trippel

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Several lines of evidence indicate that polypeptide growth factors are important in articular cartilage homeostasis and repair. It is not yet clear how these growth factors are regulated. We tested the hypothesis that the growth factors responsible for regulating cartilage are themselves regulated by growth factors. We delivered insulin-like growth factor I (IGF-I), fibroblast growth factor-2 (FGF-2), and/or transforming growth factor-β1 (TGF-β1) to adult bovine articular chondrocytes in primary culture and measured the resulting changes in IGF-I, FGF-2, and TGF-β1 gene expression and protein production. These growth factors differentially regulated their own and each others gene expression and protein production. In concert, they regulated each other in an interactive fashion. Their interactions ranged from inhibitory to synergistic. The time course of the regulatory effects differed among the individual growth factors and combinations. Growth factor-induced changes in growth factor protein production by articular chondrocytes generally corresponded to the changes in gene expression patterns. These studies suggest that interactions among IGF-I, FGF-2, and TGF-β1 substantially modulate their regulatory functions. The results may help guide the application of growth factors to articular cartilage repair.

Original languageEnglish
Pages (from-to)6697-6704
Number of pages8
JournalJournal of Biological Chemistry
Volume284
Issue number11
DOIs
StatePublished - Mar 13 2009

Fingerprint

Chondrocytes
Intercellular Signaling Peptides and Proteins
Joints
Cartilage
Transforming Growth Factors
Fibroblast Growth Factor 2
Insulin-Like Growth Factor I
Gene expression
Articular Cartilage
Gene Expression
Repair
Proteins
Homeostasis
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Growth factor regulation of growth factors in articular chondrocytes. / Shi, Shuiliang; Mercer, Scott; Eckert, George J.; Trippel, Stephen.

In: Journal of Biological Chemistry, Vol. 284, No. 11, 13.03.2009, p. 6697-6704.

Research output: Contribution to journalArticle

Shi, Shuiliang ; Mercer, Scott ; Eckert, George J. ; Trippel, Stephen. / Growth factor regulation of growth factors in articular chondrocytes. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 11. pp. 6697-6704.
@article{9d8727d0372e45fc959c93d0519722ec,
title = "Growth factor regulation of growth factors in articular chondrocytes",
abstract = "Several lines of evidence indicate that polypeptide growth factors are important in articular cartilage homeostasis and repair. It is not yet clear how these growth factors are regulated. We tested the hypothesis that the growth factors responsible for regulating cartilage are themselves regulated by growth factors. We delivered insulin-like growth factor I (IGF-I), fibroblast growth factor-2 (FGF-2), and/or transforming growth factor-β1 (TGF-β1) to adult bovine articular chondrocytes in primary culture and measured the resulting changes in IGF-I, FGF-2, and TGF-β1 gene expression and protein production. These growth factors differentially regulated their own and each others gene expression and protein production. In concert, they regulated each other in an interactive fashion. Their interactions ranged from inhibitory to synergistic. The time course of the regulatory effects differed among the individual growth factors and combinations. Growth factor-induced changes in growth factor protein production by articular chondrocytes generally corresponded to the changes in gene expression patterns. These studies suggest that interactions among IGF-I, FGF-2, and TGF-β1 substantially modulate their regulatory functions. The results may help guide the application of growth factors to articular cartilage repair.",
author = "Shuiliang Shi and Scott Mercer and Eckert, {George J.} and Stephen Trippel",
year = "2009",
month = "3",
day = "13",
doi = "10.1074/jbc.M807859200",
language = "English",
volume = "284",
pages = "6697--6704",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "11",

}

TY - JOUR

T1 - Growth factor regulation of growth factors in articular chondrocytes

AU - Shi, Shuiliang

AU - Mercer, Scott

AU - Eckert, George J.

AU - Trippel, Stephen

PY - 2009/3/13

Y1 - 2009/3/13

N2 - Several lines of evidence indicate that polypeptide growth factors are important in articular cartilage homeostasis and repair. It is not yet clear how these growth factors are regulated. We tested the hypothesis that the growth factors responsible for regulating cartilage are themselves regulated by growth factors. We delivered insulin-like growth factor I (IGF-I), fibroblast growth factor-2 (FGF-2), and/or transforming growth factor-β1 (TGF-β1) to adult bovine articular chondrocytes in primary culture and measured the resulting changes in IGF-I, FGF-2, and TGF-β1 gene expression and protein production. These growth factors differentially regulated their own and each others gene expression and protein production. In concert, they regulated each other in an interactive fashion. Their interactions ranged from inhibitory to synergistic. The time course of the regulatory effects differed among the individual growth factors and combinations. Growth factor-induced changes in growth factor protein production by articular chondrocytes generally corresponded to the changes in gene expression patterns. These studies suggest that interactions among IGF-I, FGF-2, and TGF-β1 substantially modulate their regulatory functions. The results may help guide the application of growth factors to articular cartilage repair.

AB - Several lines of evidence indicate that polypeptide growth factors are important in articular cartilage homeostasis and repair. It is not yet clear how these growth factors are regulated. We tested the hypothesis that the growth factors responsible for regulating cartilage are themselves regulated by growth factors. We delivered insulin-like growth factor I (IGF-I), fibroblast growth factor-2 (FGF-2), and/or transforming growth factor-β1 (TGF-β1) to adult bovine articular chondrocytes in primary culture and measured the resulting changes in IGF-I, FGF-2, and TGF-β1 gene expression and protein production. These growth factors differentially regulated their own and each others gene expression and protein production. In concert, they regulated each other in an interactive fashion. Their interactions ranged from inhibitory to synergistic. The time course of the regulatory effects differed among the individual growth factors and combinations. Growth factor-induced changes in growth factor protein production by articular chondrocytes generally corresponded to the changes in gene expression patterns. These studies suggest that interactions among IGF-I, FGF-2, and TGF-β1 substantially modulate their regulatory functions. The results may help guide the application of growth factors to articular cartilage repair.

UR - http://www.scopus.com/inward/record.url?scp=65449149105&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65449149105&partnerID=8YFLogxK

U2 - 10.1074/jbc.M807859200

DO - 10.1074/jbc.M807859200

M3 - Article

C2 - 19136669

AN - SCOPUS:65449149105

VL - 284

SP - 6697

EP - 6704

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 11

ER -