Growth factor stimulation of adult articular cartilage

K. D. Osborn, Stephen Trippel, H. J. Mankin

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

We have examined the effect of peptide growth factors on DNA and proteoglycan synthesis by adult bovine articular cartilage in organ culture. The actions of somatomedin-C/insulin-like growth factor I (Sm-C/IGF-I), insulin, epidermal growth factor (EGF), and fibroblast growth factor (FGF) from bovine pituitary were investigated individually and in combination. FGF stimulated a 10-fold increase in tritiated thymidine incorporation while other factors used individually did not influence mitotic activity. Used in concert, insulin with EGF and insulin with FGF acted synergistically in stimulating DNA synthesis 20-fold and 40-fold, respectively. All of these growth factors, acting individually, significantly enhanced radiosulfate incorporation. This stimulation was additive for Sm-C/IGF-I in combination with EGF or FGF, but not with insulin. These data indicate that adult bovine articular chondrocytes possess the capacity to augment both mitotic and differentiated cell functions in response to growth factors. The data further suggest that, with the exception of insulin and Sm-C/IGF-I, which appear to share a common mechanism of action, these factors produce their cellular effects via different receptor or postreceptor pathways.

Original languageEnglish (US)
Pages (from-to)35-42
Number of pages8
JournalJournal of Orthopaedic Research
Volume7
Issue number1
StatePublished - 1989
Externally publishedYes

Fingerprint

Articular Cartilage
Insulin-Like Growth Factor I
Fibroblast Growth Factors
Intercellular Signaling Peptides and Proteins
Insulin
Epidermal Growth Factor
Organ Culture Techniques
DNA
Proteoglycans
Chondrocytes
Thymidine
Joints
Peptides

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Osborn, K. D., Trippel, S., & Mankin, H. J. (1989). Growth factor stimulation of adult articular cartilage. Journal of Orthopaedic Research, 7(1), 35-42.

Growth factor stimulation of adult articular cartilage. / Osborn, K. D.; Trippel, Stephen; Mankin, H. J.

In: Journal of Orthopaedic Research, Vol. 7, No. 1, 1989, p. 35-42.

Research output: Contribution to journalArticle

Osborn, KD, Trippel, S & Mankin, HJ 1989, 'Growth factor stimulation of adult articular cartilage', Journal of Orthopaedic Research, vol. 7, no. 1, pp. 35-42.
Osborn, K. D. ; Trippel, Stephen ; Mankin, H. J. / Growth factor stimulation of adult articular cartilage. In: Journal of Orthopaedic Research. 1989 ; Vol. 7, No. 1. pp. 35-42.
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