Growth factor stimulation of hematopoietic cells leads to membrane translocation of AKT1 protein kinase

Xin Zhang, Terry A. Vik

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

AKT1 is the human homolog of the v-akt oncogene. AKT1 has two distinct protein domains, one serine/threonine kinase domain and one pleckstrin homology (PH) domain. We studied the expression and activity of AKT1 in hematopoietic cell lines. The expression of AKT1 was constitutive in hematopoietic cells of various stages of development. In the growth factor dependent MO7e cells, serum and growth factor starvation resulted in an early 50% fall in activity which was maintained over 24 h. Treatment of cells with growth factors or agents which induce differentiation activated AKT1. The subcellular localization of AKT1 in MO7e cells was altered as it was activated. High AKT1 kinase activity was associated with membrane fractions in stimulated cells, in contrast to the much lower AKT1 activity in membranes of cells starved of serum and growth factor for 1 h. These results demonstrate AKT1 kinase activity and its regulation by extracellular signaling factors in vivo in hematopoietic cells, and suggest that the activation of AKT1 involves intracellular translocation of the kinase from cytosol to membrane.

Original languageEnglish (US)
Pages (from-to)849-856
Number of pages8
JournalLeukemia Research
Volume21
Issue number9
DOIs
StatePublished - Sep 1 1997

Fingerprint

Hematopoietic Cell Growth Factors
Proto-Oncogene Proteins c-akt
Membranes
Intercellular Signaling Peptides and Proteins
Phosphotransferases
Protein-Serine-Threonine Kinases
Starvation
Serum
Oncogenes
Cytosol
Cell Membrane
Cell Line

Keywords

  • AKT1 protein kinase
  • GM-CSF
  • Hematopoiesis
  • Homology domain
  • Pleckstrin
  • Translocation

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Growth factor stimulation of hematopoietic cells leads to membrane translocation of AKT1 protein kinase. / Zhang, Xin; Vik, Terry A.

In: Leukemia Research, Vol. 21, No. 9, 01.09.1997, p. 849-856.

Research output: Contribution to journalArticle

@article{1faa9aca97f2433988b8b94a8863c0ab,
title = "Growth factor stimulation of hematopoietic cells leads to membrane translocation of AKT1 protein kinase",
abstract = "AKT1 is the human homolog of the v-akt oncogene. AKT1 has two distinct protein domains, one serine/threonine kinase domain and one pleckstrin homology (PH) domain. We studied the expression and activity of AKT1 in hematopoietic cell lines. The expression of AKT1 was constitutive in hematopoietic cells of various stages of development. In the growth factor dependent MO7e cells, serum and growth factor starvation resulted in an early 50{\%} fall in activity which was maintained over 24 h. Treatment of cells with growth factors or agents which induce differentiation activated AKT1. The subcellular localization of AKT1 in MO7e cells was altered as it was activated. High AKT1 kinase activity was associated with membrane fractions in stimulated cells, in contrast to the much lower AKT1 activity in membranes of cells starved of serum and growth factor for 1 h. These results demonstrate AKT1 kinase activity and its regulation by extracellular signaling factors in vivo in hematopoietic cells, and suggest that the activation of AKT1 involves intracellular translocation of the kinase from cytosol to membrane.",
keywords = "AKT1 protein kinase, GM-CSF, Hematopoiesis, Homology domain, Pleckstrin, Translocation",
author = "Xin Zhang and Vik, {Terry A.}",
year = "1997",
month = "9",
day = "1",
doi = "10.1016/S0145-2126(97)00055-6",
language = "English (US)",
volume = "21",
pages = "849--856",
journal = "Leukemia Research",
issn = "0145-2126",
publisher = "Elsevier Limited",
number = "9",

}

TY - JOUR

T1 - Growth factor stimulation of hematopoietic cells leads to membrane translocation of AKT1 protein kinase

AU - Zhang, Xin

AU - Vik, Terry A.

PY - 1997/9/1

Y1 - 1997/9/1

N2 - AKT1 is the human homolog of the v-akt oncogene. AKT1 has two distinct protein domains, one serine/threonine kinase domain and one pleckstrin homology (PH) domain. We studied the expression and activity of AKT1 in hematopoietic cell lines. The expression of AKT1 was constitutive in hematopoietic cells of various stages of development. In the growth factor dependent MO7e cells, serum and growth factor starvation resulted in an early 50% fall in activity which was maintained over 24 h. Treatment of cells with growth factors or agents which induce differentiation activated AKT1. The subcellular localization of AKT1 in MO7e cells was altered as it was activated. High AKT1 kinase activity was associated with membrane fractions in stimulated cells, in contrast to the much lower AKT1 activity in membranes of cells starved of serum and growth factor for 1 h. These results demonstrate AKT1 kinase activity and its regulation by extracellular signaling factors in vivo in hematopoietic cells, and suggest that the activation of AKT1 involves intracellular translocation of the kinase from cytosol to membrane.

AB - AKT1 is the human homolog of the v-akt oncogene. AKT1 has two distinct protein domains, one serine/threonine kinase domain and one pleckstrin homology (PH) domain. We studied the expression and activity of AKT1 in hematopoietic cell lines. The expression of AKT1 was constitutive in hematopoietic cells of various stages of development. In the growth factor dependent MO7e cells, serum and growth factor starvation resulted in an early 50% fall in activity which was maintained over 24 h. Treatment of cells with growth factors or agents which induce differentiation activated AKT1. The subcellular localization of AKT1 in MO7e cells was altered as it was activated. High AKT1 kinase activity was associated with membrane fractions in stimulated cells, in contrast to the much lower AKT1 activity in membranes of cells starved of serum and growth factor for 1 h. These results demonstrate AKT1 kinase activity and its regulation by extracellular signaling factors in vivo in hematopoietic cells, and suggest that the activation of AKT1 involves intracellular translocation of the kinase from cytosol to membrane.

KW - AKT1 protein kinase

KW - GM-CSF

KW - Hematopoiesis

KW - Homology domain

KW - Pleckstrin

KW - Translocation

UR - http://www.scopus.com/inward/record.url?scp=0030669730&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030669730&partnerID=8YFLogxK

U2 - 10.1016/S0145-2126(97)00055-6

DO - 10.1016/S0145-2126(97)00055-6

M3 - Article

C2 - 9393600

AN - SCOPUS:0030669730

VL - 21

SP - 849

EP - 856

JO - Leukemia Research

JF - Leukemia Research

SN - 0145-2126

IS - 9

ER -