Growth hormone-releasing factor (GRF) regulates expression of its own receptor

Reiko Horikawa, Pattie Hellmann, Silvano G. Cella, Antonio Torsello, Richard Day, Eugenio E. Muller, Michael O. Thorner

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Recent studies have demonstrated that passive immunization of neonatal rats to GRF inhibited their somatic growth through the suppression of GH secretion. In this study, we investigated the changes in pituitary GRF receptor (GRFR) expression in GRF antibody (GRF-ab) treated rats. Neonatal rats were treated from day 1 to day 10 after birth with every other day sc injection of 50 μl of normal rabbit serum (groups I: control and III) or rabbit serum containing GRF-ab (groups II and IV). In addition, groups III and IV received twice daily injection of recombinant human GH (0.4 μg/kg, sc). The rats were sacrificed on day 11 and pituitaries were removed. The pituitary weights in all treatment groups were decreased compared to the control group (1). Total pituitary RNA was extracted and GRFR mRNA levels were determined by RNase protection assay. Receptor RNA levels were quantitated and normalized to an internal standard, glyceraldehyde 3- phosphate dehydrogenase (GAPDH). The ratios of GRFR mRNA to GAPDH mRNA were significantly decreased to 49.6 ± 4.9 (mean ± SD), 73.0 ± 8.7, 43.6 ± 9.5% of control group I in the experimental groups II, III, and IV, respectively (P

Original languageEnglish (US)
Pages (from-to)2642-2645
Number of pages4
JournalEndocrinology
Volume137
Issue number6
DOIs
StatePublished - 1996
Externally publishedYes

Fingerprint

Growth Hormone-Releasing Hormone
Glyceraldehyde-3-Phosphate Dehydrogenases
Control Groups
Messenger RNA
RNA
Rabbits
Injections
Passive Immunization
Ribonucleases
Serum
Growth Hormone
Parturition
Weights and Measures
Antibodies
Growth
Therapeutics

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Horikawa, R., Hellmann, P., Cella, S. G., Torsello, A., Day, R., Muller, E. E., & Thorner, M. O. (1996). Growth hormone-releasing factor (GRF) regulates expression of its own receptor. Endocrinology, 137(6), 2642-2645. https://doi.org/10.1210/en.137.6.2642

Growth hormone-releasing factor (GRF) regulates expression of its own receptor. / Horikawa, Reiko; Hellmann, Pattie; Cella, Silvano G.; Torsello, Antonio; Day, Richard; Muller, Eugenio E.; Thorner, Michael O.

In: Endocrinology, Vol. 137, No. 6, 1996, p. 2642-2645.

Research output: Contribution to journalArticle

Horikawa, R, Hellmann, P, Cella, SG, Torsello, A, Day, R, Muller, EE & Thorner, MO 1996, 'Growth hormone-releasing factor (GRF) regulates expression of its own receptor', Endocrinology, vol. 137, no. 6, pp. 2642-2645. https://doi.org/10.1210/en.137.6.2642
Horikawa R, Hellmann P, Cella SG, Torsello A, Day R, Muller EE et al. Growth hormone-releasing factor (GRF) regulates expression of its own receptor. Endocrinology. 1996;137(6):2642-2645. https://doi.org/10.1210/en.137.6.2642
Horikawa, Reiko ; Hellmann, Pattie ; Cella, Silvano G. ; Torsello, Antonio ; Day, Richard ; Muller, Eugenio E. ; Thorner, Michael O. / Growth hormone-releasing factor (GRF) regulates expression of its own receptor. In: Endocrinology. 1996 ; Vol. 137, No. 6. pp. 2642-2645.
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