Abstract
Growth hormone releasing factor (GHRF) was determined by radio-immunoassay in cerebrospinal fluid (CSF) from 37 unmedicated healthy volunteers (mean ± SD = 23.4 ± 7.2 pg/ml). A second lumbar puncture in 11 of these subjects suggested that the CSF GHRF levels were stable over time. High performance liquid chromatography revealed that the CSF immunoreactivity co-eluted with the 1-44NH2 and 1-4ONH2 forms of GHRF. Measurements of GHRF in sequential CSF aliquots revealed no evidence of a rostro-caudal gradient, an observation that is compatible with the hypothesis that GHRF has a long half-life in CSF. A significant negative correlation with body weight was found in the 37 subjects. Levels of CSF GHRF were determined in 24 lithium-treated euthymic bipolar patients, 13 of whom provided a second CSF sample during the unmedicated state. No group differences or effects of lithium were noted.
| Original language | English |
|---|---|
| Pages (from-to) | 141-147 |
| Number of pages | 7 |
| Journal | Psychiatry Research |
| Volume | 22 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1987 |
| Externally published | Yes |
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Keywords
- cerebrospinal fluid
- Growth hormone releasing factor
- manic-depressive illness
ASJC Scopus subject areas
- Psychiatry and Mental health
- Biological Psychiatry
- Psychology(all)
Cite this
Growth hormone releasing factor in human cerebrospinal fluid. / Berrettini, Wade H.; Nurnberger, John; Simmons-Alling, Susan.
In: Psychiatry Research, Vol. 22, No. 2, 1987, p. 141-147.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Growth hormone releasing factor in human cerebrospinal fluid
AU - Berrettini, Wade H.
AU - Nurnberger, John
AU - Simmons-Alling, Susan
PY - 1987
Y1 - 1987
N2 - Growth hormone releasing factor (GHRF) was determined by radio-immunoassay in cerebrospinal fluid (CSF) from 37 unmedicated healthy volunteers (mean ± SD = 23.4 ± 7.2 pg/ml). A second lumbar puncture in 11 of these subjects suggested that the CSF GHRF levels were stable over time. High performance liquid chromatography revealed that the CSF immunoreactivity co-eluted with the 1-44NH2 and 1-4ONH2 forms of GHRF. Measurements of GHRF in sequential CSF aliquots revealed no evidence of a rostro-caudal gradient, an observation that is compatible with the hypothesis that GHRF has a long half-life in CSF. A significant negative correlation with body weight was found in the 37 subjects. Levels of CSF GHRF were determined in 24 lithium-treated euthymic bipolar patients, 13 of whom provided a second CSF sample during the unmedicated state. No group differences or effects of lithium were noted.
AB - Growth hormone releasing factor (GHRF) was determined by radio-immunoassay in cerebrospinal fluid (CSF) from 37 unmedicated healthy volunteers (mean ± SD = 23.4 ± 7.2 pg/ml). A second lumbar puncture in 11 of these subjects suggested that the CSF GHRF levels were stable over time. High performance liquid chromatography revealed that the CSF immunoreactivity co-eluted with the 1-44NH2 and 1-4ONH2 forms of GHRF. Measurements of GHRF in sequential CSF aliquots revealed no evidence of a rostro-caudal gradient, an observation that is compatible with the hypothesis that GHRF has a long half-life in CSF. A significant negative correlation with body weight was found in the 37 subjects. Levels of CSF GHRF were determined in 24 lithium-treated euthymic bipolar patients, 13 of whom provided a second CSF sample during the unmedicated state. No group differences or effects of lithium were noted.
KW - cerebrospinal fluid
KW - Growth hormone releasing factor
KW - manic-depressive illness
UR - http://www.scopus.com/inward/record.url?scp=0023618070&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023618070&partnerID=8YFLogxK
U2 - 10.1016/0165-1781(87)90101-6
DO - 10.1016/0165-1781(87)90101-6
M3 - Article
VL - 22
SP - 141
EP - 147
JO - Psychiatry Research
JF - Psychiatry Research
SN - 0165-1781
IS - 2
ER -