Growth Inhibition of a Human Colorectal Carcinoma Cell Line by Interleukin 1 Is Associated with Enhanced Expression of γ-Interferon Receptors

Arthur B. Raitano, Murray Korc

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31 Scopus citations

Abstract

Recombinant human tumor necrosis factor and recombinant human γ interferon (IFN-7) exert synergistic growth inhibitory effects in WiDR human colorectal carcinoma cells. In this cell line, tumor necrosis factor increases IFN-7 binding. Interleukin 1 (IL-1) is a cytokine that mimics many of the biological actions of TNF. Therefore, in the present study, we investigated the effects of recombinant human IL-1 on cell growth and IFN-7 receptor expression in WiDR cells. IL-1 slightly inhibited the growth of WiDR cells, and exerted additive growth inhibitory effects in the presence of IFN-γ. IL-1 caused a time- and dose-dependent increase in 125I-labeled IFN-7 binding that was maximal at 6 h, persisted for at least 24 h, and was blocked by both actinomycin D and cycloheximide. The increase in binding was associated with an increase in cell surface IFN-7 receptor protein expression as determined by Scatchard analysis of equilibrium binding data and by immunofluorescent staining with an anti-human IFN-7 receptor monoclonal antibody. IL-1 also produced a time-and dose-dependent increase in IFN-7 receptor mRNA levels that was maximal at 3 h and persisted for at least 24 h. Actinomycin D, but not cycloheximide, completely blocked the IL-1-mediated increase in IFN-7 receptor mRNA levels. However, IL-1 did not alter IFN-7 receptor mRNA half-life. These data indicate that IL-1 and IFN-7 exert additive growth inhibitory effects on colon cancer cell growth, and suggest that IL-1 increases IFN-7 receptor expression in these cells by enhancing IFN-7 mRNA levels.

Original languageEnglish (US)
Pages (from-to)636-640
Number of pages5
JournalCancer Research
Volume53
Issue number3
StatePublished - Feb 1993

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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