Growth of human nondiploid primary prostate tumor epithelial cells in vitro

Dharam P. Chopra, Fazlul H. Sarkar, David Grignon, Wael A. Sakr, Anwar Mohamed, Anuradha Waghray

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Research into molecular and cellular defects underlying prostate cancer would be advanced by in vitro models of prostate tumor cells representing patient tumors. We have propagated, in serum-free medium, epithelial cell cultures derived from nondiploid prostate tumors and normal human prostate. The serial passage tumor cells exhibited nondiploid karyotype and transformed phenotypes of focus formation and anchorage-independent growth. In contrast, the normal prostate cells showed diploid karyotype and lacked transformed phenotypes. Both the tumor and normal cells were positive for prostate- specific antigen and cytokeratins 18 and 19 and negative for keratin 15. These results demonstrate that the nondiploid prostate tumors and normal prostate epithelial cell cultures retained their respective in vivo properties and should allow studies to elucidate molecular alterations involved in human prostate cancer.

Original languageEnglish (US)
Pages (from-to)3688-3692
Number of pages5
JournalCancer Research
Volume57
Issue number17
StatePublished - Sep 1 1997
Externally publishedYes

Fingerprint

Prostate
Epithelial Cells
Growth
Neoplasms
Karyotype
Keratin-15
Prostatic Neoplasms
Cell Culture Techniques
Keratin-18
Keratin-19
Serial Passage
Phenotype
Serum-Free Culture Media
Prostate-Specific Antigen
Diploidy
In Vitro Techniques
Research

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Chopra, D. P., Sarkar, F. H., Grignon, D., Sakr, W. A., Mohamed, A., & Waghray, A. (1997). Growth of human nondiploid primary prostate tumor epithelial cells in vitro. Cancer Research, 57(17), 3688-3692.

Growth of human nondiploid primary prostate tumor epithelial cells in vitro. / Chopra, Dharam P.; Sarkar, Fazlul H.; Grignon, David; Sakr, Wael A.; Mohamed, Anwar; Waghray, Anuradha.

In: Cancer Research, Vol. 57, No. 17, 01.09.1997, p. 3688-3692.

Research output: Contribution to journalArticle

Chopra, DP, Sarkar, FH, Grignon, D, Sakr, WA, Mohamed, A & Waghray, A 1997, 'Growth of human nondiploid primary prostate tumor epithelial cells in vitro', Cancer Research, vol. 57, no. 17, pp. 3688-3692.
Chopra DP, Sarkar FH, Grignon D, Sakr WA, Mohamed A, Waghray A. Growth of human nondiploid primary prostate tumor epithelial cells in vitro. Cancer Research. 1997 Sep 1;57(17):3688-3692.
Chopra, Dharam P. ; Sarkar, Fazlul H. ; Grignon, David ; Sakr, Wael A. ; Mohamed, Anwar ; Waghray, Anuradha. / Growth of human nondiploid primary prostate tumor epithelial cells in vitro. In: Cancer Research. 1997 ; Vol. 57, No. 17. pp. 3688-3692.
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