Abstract
Research into molecular and cellular defects underlying prostate cancer would be advanced by in vitro models of prostate tumor cells representing patient tumors. We have propagated, in serum-free medium, epithelial cell cultures derived from nondiploid prostate tumors and normal human prostate. The serial passage tumor cells exhibited nondiploid karyotype and transformed phenotypes of focus formation and anchorage-independent growth. In contrast, the normal prostate cells showed diploid karyotype and lacked transformed phenotypes. Both the tumor and normal cells were positive for prostate- specific antigen and cytokeratins 18 and 19 and negative for keratin 15. These results demonstrate that the nondiploid prostate tumors and normal prostate epithelial cell cultures retained their respective in vivo properties and should allow studies to elucidate molecular alterations involved in human prostate cancer.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3688-3692 |
| Number of pages | 5 |
| Journal | Cancer Research |
| Volume | 57 |
| Issue number | 17 |
| State | Published - Sep 1 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Growth of human nondiploid primary prostate tumor epithelial cells in vitro. / Chopra, Dharam P.; Sarkar, Fazlul H.; Grignon, David; Sakr, Wael A.; Mohamed, Anwar; Waghray, Anuradha.
In: Cancer Research, Vol. 57, No. 17, 01.09.1997, p. 3688-3692.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Growth of human nondiploid primary prostate tumor epithelial cells in vitro
AU - Chopra, Dharam P.
AU - Sarkar, Fazlul H.
AU - Grignon, David
AU - Sakr, Wael A.
AU - Mohamed, Anwar
AU - Waghray, Anuradha
PY - 1997/9/1
Y1 - 1997/9/1
N2 - Research into molecular and cellular defects underlying prostate cancer would be advanced by in vitro models of prostate tumor cells representing patient tumors. We have propagated, in serum-free medium, epithelial cell cultures derived from nondiploid prostate tumors and normal human prostate. The serial passage tumor cells exhibited nondiploid karyotype and transformed phenotypes of focus formation and anchorage-independent growth. In contrast, the normal prostate cells showed diploid karyotype and lacked transformed phenotypes. Both the tumor and normal cells were positive for prostate- specific antigen and cytokeratins 18 and 19 and negative for keratin 15. These results demonstrate that the nondiploid prostate tumors and normal prostate epithelial cell cultures retained their respective in vivo properties and should allow studies to elucidate molecular alterations involved in human prostate cancer.
AB - Research into molecular and cellular defects underlying prostate cancer would be advanced by in vitro models of prostate tumor cells representing patient tumors. We have propagated, in serum-free medium, epithelial cell cultures derived from nondiploid prostate tumors and normal human prostate. The serial passage tumor cells exhibited nondiploid karyotype and transformed phenotypes of focus formation and anchorage-independent growth. In contrast, the normal prostate cells showed diploid karyotype and lacked transformed phenotypes. Both the tumor and normal cells were positive for prostate- specific antigen and cytokeratins 18 and 19 and negative for keratin 15. These results demonstrate that the nondiploid prostate tumors and normal prostate epithelial cell cultures retained their respective in vivo properties and should allow studies to elucidate molecular alterations involved in human prostate cancer.
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UR - http://www.scopus.com/inward/citedby.url?scp=0030874837&partnerID=8YFLogxK
M3 - Article
C2 - 9288774
AN - SCOPUS:0030874837
VL - 57
SP - 3688
EP - 3692
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0099-7013
IS - 17
ER -