Growth of molluscum contagiosum virus in a human foreskin xenograft model

Kenneth Fife, Margot Whitfeld, Holly Faust, Michael P. Goheen, Janine Bryan, Darron Brown

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Molluscum contagiosum virus (MCV) is a common pathogen causing a troublesome skin condition in many immunocompetent Individuals and a widespread, disfiguring affliction in many patients with AIDS. We have successfully infected human foreskin fragments with a patient-derived isolate of MCV. This was demonstrated by exposing the foreskin pieces to a patient lesion extract and implanting the tissue under the renal capsule of athymic mice. Light and electron microscopic examination of infected implants showed the presence of cytoplasmic inclusions containing typical poxvirus particles within 2-3 weeks of implantation. Replication of MCV was established by demonstrating that viable virus was required to produce the cytopathologic effects, and viral DNA replication was demonstrated by incorporation of bromodeoxyuridine into cytoplasmic inclusions. Four additional patient extracts (representing both described MCV types) were also used to successfully infect foreskin implants. A limited number of attempts to pass virus from one infected implant to another were not successful. This system is the most rapid and reproducible for growing MCV that has been reported to date.

Original languageEnglish
Pages (from-to)95-101
Number of pages7
JournalVirology
Volume226
Issue number1
DOIs
StatePublished - Dec 1 1996

Fingerprint

Molluscum contagiosum virus
Foreskin
Heterografts
Growth
Inclusion Bodies
Viruses
Poxviridae
Tissue Extracts
Viral DNA
Bromodeoxyuridine
DNA Replication
Nude Mice
Capsules
Acquired Immunodeficiency Syndrome
Electrons
Kidney
Light
Skin

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Growth of molluscum contagiosum virus in a human foreskin xenograft model. / Fife, Kenneth; Whitfeld, Margot; Faust, Holly; Goheen, Michael P.; Bryan, Janine; Brown, Darron.

In: Virology, Vol. 226, No. 1, 01.12.1996, p. 95-101.

Research output: Contribution to journalArticle

Fife, Kenneth ; Whitfeld, Margot ; Faust, Holly ; Goheen, Michael P. ; Bryan, Janine ; Brown, Darron. / Growth of molluscum contagiosum virus in a human foreskin xenograft model. In: Virology. 1996 ; Vol. 226, No. 1. pp. 95-101.
@article{09c0068b2ed848638fb51abc912b9a7c,
title = "Growth of molluscum contagiosum virus in a human foreskin xenograft model",
abstract = "Molluscum contagiosum virus (MCV) is a common pathogen causing a troublesome skin condition in many immunocompetent Individuals and a widespread, disfiguring affliction in many patients with AIDS. We have successfully infected human foreskin fragments with a patient-derived isolate of MCV. This was demonstrated by exposing the foreskin pieces to a patient lesion extract and implanting the tissue under the renal capsule of athymic mice. Light and electron microscopic examination of infected implants showed the presence of cytoplasmic inclusions containing typical poxvirus particles within 2-3 weeks of implantation. Replication of MCV was established by demonstrating that viable virus was required to produce the cytopathologic effects, and viral DNA replication was demonstrated by incorporation of bromodeoxyuridine into cytoplasmic inclusions. Four additional patient extracts (representing both described MCV types) were also used to successfully infect foreskin implants. A limited number of attempts to pass virus from one infected implant to another were not successful. This system is the most rapid and reproducible for growing MCV that has been reported to date.",
author = "Kenneth Fife and Margot Whitfeld and Holly Faust and Goheen, {Michael P.} and Janine Bryan and Darron Brown",
year = "1996",
month = "12",
day = "1",
doi = "10.1006/viro.1996.0631",
language = "English",
volume = "226",
pages = "95--101",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Growth of molluscum contagiosum virus in a human foreskin xenograft model

AU - Fife, Kenneth

AU - Whitfeld, Margot

AU - Faust, Holly

AU - Goheen, Michael P.

AU - Bryan, Janine

AU - Brown, Darron

PY - 1996/12/1

Y1 - 1996/12/1

N2 - Molluscum contagiosum virus (MCV) is a common pathogen causing a troublesome skin condition in many immunocompetent Individuals and a widespread, disfiguring affliction in many patients with AIDS. We have successfully infected human foreskin fragments with a patient-derived isolate of MCV. This was demonstrated by exposing the foreskin pieces to a patient lesion extract and implanting the tissue under the renal capsule of athymic mice. Light and electron microscopic examination of infected implants showed the presence of cytoplasmic inclusions containing typical poxvirus particles within 2-3 weeks of implantation. Replication of MCV was established by demonstrating that viable virus was required to produce the cytopathologic effects, and viral DNA replication was demonstrated by incorporation of bromodeoxyuridine into cytoplasmic inclusions. Four additional patient extracts (representing both described MCV types) were also used to successfully infect foreskin implants. A limited number of attempts to pass virus from one infected implant to another were not successful. This system is the most rapid and reproducible for growing MCV that has been reported to date.

AB - Molluscum contagiosum virus (MCV) is a common pathogen causing a troublesome skin condition in many immunocompetent Individuals and a widespread, disfiguring affliction in many patients with AIDS. We have successfully infected human foreskin fragments with a patient-derived isolate of MCV. This was demonstrated by exposing the foreskin pieces to a patient lesion extract and implanting the tissue under the renal capsule of athymic mice. Light and electron microscopic examination of infected implants showed the presence of cytoplasmic inclusions containing typical poxvirus particles within 2-3 weeks of implantation. Replication of MCV was established by demonstrating that viable virus was required to produce the cytopathologic effects, and viral DNA replication was demonstrated by incorporation of bromodeoxyuridine into cytoplasmic inclusions. Four additional patient extracts (representing both described MCV types) were also used to successfully infect foreskin implants. A limited number of attempts to pass virus from one infected implant to another were not successful. This system is the most rapid and reproducible for growing MCV that has been reported to date.

UR - http://www.scopus.com/inward/record.url?scp=0030560808&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030560808&partnerID=8YFLogxK

U2 - 10.1006/viro.1996.0631

DO - 10.1006/viro.1996.0631

M3 - Article

VL - 226

SP - 95

EP - 101

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -