Growth stage dependent effects of electromagnetic fields on DNA synthesis of Jurkat cells

G. Nindl, J. A. Swez, J. M. Miller, W. X. Balcavage

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A 1.8 mT, bone healing, electromagnetic field (EMF) and power frequency EMFs of 0.1 and 0.4 mT significantly inhibit DNA synthesis in otherwise unstimulated Jurkat (E 6.1) cells. Inhibition is generally most prominent in cells from mid log phase growth. In complete medium the bone healing EMF inhibits [3H] thymidine uptake of the latter cells by almost 50% vs. 20-25% inhibition by 60 Hz fields. Cells in conditioned medium are even more sensitive to EMFs with inhibition up to ca. 60%. The effects of the 0.1 and 0.4 mT power frequency EMFs mere very similar suggesting saturation at 0.1 mT or lower.

Original languageEnglish
Pages (from-to)501-506
Number of pages6
JournalFEBS Letters
Volume414
Issue number3
DOIs
StatePublished - Sep 15 1997

Fingerprint

Electromagnetic Fields
Jurkat Cells
Electromagnetic fields
DNA
Bone
Electric potential
Growth
Conditioned Culture Medium
Bone and Bones
Thymidine

Keywords

  • DNA synthesis
  • Electromagnetic field
  • Growth
  • Jurkat T-cell line
  • T-lymphocyte

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Growth stage dependent effects of electromagnetic fields on DNA synthesis of Jurkat cells. / Nindl, G.; Swez, J. A.; Miller, J. M.; Balcavage, W. X.

In: FEBS Letters, Vol. 414, No. 3, 15.09.1997, p. 501-506.

Research output: Contribution to journalArticle

Nindl, G. ; Swez, J. A. ; Miller, J. M. ; Balcavage, W. X. / Growth stage dependent effects of electromagnetic fields on DNA synthesis of Jurkat cells. In: FEBS Letters. 1997 ; Vol. 414, No. 3. pp. 501-506.
@article{f829eabbab7746f5abe41615d5980a8c,
title = "Growth stage dependent effects of electromagnetic fields on DNA synthesis of Jurkat cells",
abstract = "A 1.8 mT, bone healing, electromagnetic field (EMF) and power frequency EMFs of 0.1 and 0.4 mT significantly inhibit DNA synthesis in otherwise unstimulated Jurkat (E 6.1) cells. Inhibition is generally most prominent in cells from mid log phase growth. In complete medium the bone healing EMF inhibits [3H] thymidine uptake of the latter cells by almost 50{\%} vs. 20-25{\%} inhibition by 60 Hz fields. Cells in conditioned medium are even more sensitive to EMFs with inhibition up to ca. 60{\%}. The effects of the 0.1 and 0.4 mT power frequency EMFs mere very similar suggesting saturation at 0.1 mT or lower.",
keywords = "DNA synthesis, Electromagnetic field, Growth, Jurkat T-cell line, T-lymphocyte",
author = "G. Nindl and Swez, {J. A.} and Miller, {J. M.} and Balcavage, {W. X.}",
year = "1997",
month = "9",
day = "15",
doi = "10.1016/S0014-5793(97)01060-0",
language = "English",
volume = "414",
pages = "501--506",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Growth stage dependent effects of electromagnetic fields on DNA synthesis of Jurkat cells

AU - Nindl, G.

AU - Swez, J. A.

AU - Miller, J. M.

AU - Balcavage, W. X.

PY - 1997/9/15

Y1 - 1997/9/15

N2 - A 1.8 mT, bone healing, electromagnetic field (EMF) and power frequency EMFs of 0.1 and 0.4 mT significantly inhibit DNA synthesis in otherwise unstimulated Jurkat (E 6.1) cells. Inhibition is generally most prominent in cells from mid log phase growth. In complete medium the bone healing EMF inhibits [3H] thymidine uptake of the latter cells by almost 50% vs. 20-25% inhibition by 60 Hz fields. Cells in conditioned medium are even more sensitive to EMFs with inhibition up to ca. 60%. The effects of the 0.1 and 0.4 mT power frequency EMFs mere very similar suggesting saturation at 0.1 mT or lower.

AB - A 1.8 mT, bone healing, electromagnetic field (EMF) and power frequency EMFs of 0.1 and 0.4 mT significantly inhibit DNA synthesis in otherwise unstimulated Jurkat (E 6.1) cells. Inhibition is generally most prominent in cells from mid log phase growth. In complete medium the bone healing EMF inhibits [3H] thymidine uptake of the latter cells by almost 50% vs. 20-25% inhibition by 60 Hz fields. Cells in conditioned medium are even more sensitive to EMFs with inhibition up to ca. 60%. The effects of the 0.1 and 0.4 mT power frequency EMFs mere very similar suggesting saturation at 0.1 mT or lower.

KW - DNA synthesis

KW - Electromagnetic field

KW - Growth

KW - Jurkat T-cell line

KW - T-lymphocyte

UR - http://www.scopus.com/inward/record.url?scp=0030767655&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030767655&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(97)01060-0

DO - 10.1016/S0014-5793(97)01060-0

M3 - Article

C2 - 9323024

AN - SCOPUS:0030767655

VL - 414

SP - 501

EP - 506

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -