GRP94 resides within cardiac sarcoplasmic reticulum vesicles and is phosphorylated by casein kinase II

Steven E. Cala, Larry Jones

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Cardiac sarcoplasmic reticulum (SR) plays a dominant role in cellular Ca2+ homeostasis by storing and releasing Ca2+. SDS-polyacrylamide gel electrophoresis and Stains All staining reveals that at least six Ca2+- binding proteins are contained in cardiac SR vesicles, five of which have now been identified. These five SR proteins comprise a set of high capacity Ca2+-binding proteins, localized to the SR lumen, that exhibit properties expected for physiological Ca2+ stores. In this study, we have purified and isolated cDNA clones for the sixth major Stains All blue-staining protein of dog cardiac SR and identified it as GRP94 (glucose-regulated protein, M(r) = 94,000). Previously, this prominent Ca2+-binding component has only been described in non-muscle endoplasmic reticulum. Cardiac GRP94 co-sedimented with cardiac SR vesicles and all previously described SR markers and was completely contained within the SR lumen. GRP94, like several other SR Ca2+-binding proteins, was a substrate for casein kinase II and was phosphorylated at two or more sites located near the two ends of the molecule. A low level of endogenous casein kinase II activity was found in crude preparations of cardiac SR but did not co-purify with SR vesicles after calcium oxalate loading, suggesting that casein kinase II phosphorylation in vivo occurs at a site other than the SR.

Original languageEnglish
Pages (from-to)5926-5931
Number of pages6
JournalJournal of Biological Chemistry
Volume269
Issue number8
StatePublished - Feb 25 1994

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Casein Kinase II
Sarcoplasmic Reticulum
Carrier Proteins
Calcium Oxalate
Phosphorylation
Electrophoresis
Proteins
Complementary DNA
Molecules
glucose-regulated proteins
Substrates
Staining and Labeling
Endoplasmic Reticulum
stains-all
Polyacrylamide Gel Electrophoresis
Homeostasis

ASJC Scopus subject areas

  • Biochemistry

Cite this

GRP94 resides within cardiac sarcoplasmic reticulum vesicles and is phosphorylated by casein kinase II. / Cala, Steven E.; Jones, Larry.

In: Journal of Biological Chemistry, Vol. 269, No. 8, 25.02.1994, p. 5926-5931.

Research output: Contribution to journalArticle

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