Guiding principles of specimen preservation for confocal fluorescence microscopy

Research output: Chapter in Book/Report/Conference proceedingChapter

32 Citations (Scopus)

Abstract

Traditionally, biologists have been confined to transmission electron microscopy (TEM) and light microscopy (LM) in order to correlate biochemical and molecular data with morphology. Electron microscopy (EM) provides fine ultrastructural detail but is limited to the study of cellular structures that react with electron dense stains deposited in fixed specimens. Immunogold labeling permits the study of non-electron-dense material, but EM sections must still be very thin to avoid problems with the penetration of the labeled antibodies and to reduce scattering of the electron beam.

Original languageEnglish
Title of host publicationHandbook of Biological Confocal Microscopy: Third Edition
PublisherSpringer US
Pages368-380
Number of pages13
ISBN (Print)038725921X, 9780387259215
DOIs
StatePublished - 2006

Fingerprint

fluorescence microscopy
Fluorescence Microscopy
Confocal Microscopy
electron microscopy
Electron Microscopy
electrons
Electrons
cell structures
Cellular Structures
Transmission Electron Microscopy
biologists
transmission electron microscopy
light microscopy
Microscopy
Coloring Agents
Light
antibodies
Antibodies
confocal microscopy

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Bacallao, R., Sohrab, S., & Phillips, C. (2006). Guiding principles of specimen preservation for confocal fluorescence microscopy. In Handbook of Biological Confocal Microscopy: Third Edition (pp. 368-380). Springer US. https://doi.org/10.1007/978-0-387-45524-2_18

Guiding principles of specimen preservation for confocal fluorescence microscopy. / Bacallao, Robert; Sohrab, Sadaf; Phillips, Carrie.

Handbook of Biological Confocal Microscopy: Third Edition. Springer US, 2006. p. 368-380.

Research output: Chapter in Book/Report/Conference proceedingChapter

Bacallao, R, Sohrab, S & Phillips, C 2006, Guiding principles of specimen preservation for confocal fluorescence microscopy. in Handbook of Biological Confocal Microscopy: Third Edition. Springer US, pp. 368-380. https://doi.org/10.1007/978-0-387-45524-2_18
Bacallao R, Sohrab S, Phillips C. Guiding principles of specimen preservation for confocal fluorescence microscopy. In Handbook of Biological Confocal Microscopy: Third Edition. Springer US. 2006. p. 368-380 https://doi.org/10.1007/978-0-387-45524-2_18
Bacallao, Robert ; Sohrab, Sadaf ; Phillips, Carrie. / Guiding principles of specimen preservation for confocal fluorescence microscopy. Handbook of Biological Confocal Microscopy: Third Edition. Springer US, 2006. pp. 368-380
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