Abstract
Traditionally, biologists have been confined to transmission electron microscopy (TEM) and light microscopy (LM) in order to correlate biochemical and molecular data with morphology. Electron microscopy (EM) provides fine ultrastructural detail but is limited to the study of cellular structures that react with electron dense stains deposited in fixed specimens. Immunogold labeling permits the study of non-electron-dense material, but EM sections must still be very thin to avoid problems with the penetration of the labeled antibodies and to reduce scattering of the electron beam.
| Original language | English (US) |
|---|---|
| Title of host publication | Handbook of Biological Confocal Microscopy |
| Subtitle of host publication | Third Edition |
| Publisher | Springer US |
| Pages | 368-380 |
| Number of pages | 13 |
| ISBN (Print) | 038725921X, 9780387259215 |
| DOIs | |
| State | Published - Dec 1 2006 |
Fingerprint
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
Cite this
Guiding principles of specimen preservation for confocal fluorescence microscopy. / Bacallao, Robert; Sohrab, Sadaf; Phillips, Carrie.
Handbook of Biological Confocal Microscopy: Third Edition. Springer US, 2006. p. 368-380.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Guiding principles of specimen preservation for confocal fluorescence microscopy
AU - Bacallao, Robert
AU - Sohrab, Sadaf
AU - Phillips, Carrie
PY - 2006/12/1
Y1 - 2006/12/1
N2 - Traditionally, biologists have been confined to transmission electron microscopy (TEM) and light microscopy (LM) in order to correlate biochemical and molecular data with morphology. Electron microscopy (EM) provides fine ultrastructural detail but is limited to the study of cellular structures that react with electron dense stains deposited in fixed specimens. Immunogold labeling permits the study of non-electron-dense material, but EM sections must still be very thin to avoid problems with the penetration of the labeled antibodies and to reduce scattering of the electron beam.
AB - Traditionally, biologists have been confined to transmission electron microscopy (TEM) and light microscopy (LM) in order to correlate biochemical and molecular data with morphology. Electron microscopy (EM) provides fine ultrastructural detail but is limited to the study of cellular structures that react with electron dense stains deposited in fixed specimens. Immunogold labeling permits the study of non-electron-dense material, but EM sections must still be very thin to avoid problems with the penetration of the labeled antibodies and to reduce scattering of the electron beam.
UR - http://www.scopus.com/inward/record.url?scp=57349128221&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=57349128221&partnerID=8YFLogxK
U2 - 10.1007/978-0-387-45524-2_18
DO - 10.1007/978-0-387-45524-2_18
M3 - Chapter
AN - SCOPUS:57349128221
SN - 038725921X
SN - 9780387259215
SP - 368
EP - 380
BT - Handbook of Biological Confocal Microscopy
PB - Springer US
ER -