Gut microbiota was modulated by moxibustion stimulation in rats with irritable bowel syndrome 11 Medical and Health Sciences 1109 Neurosciences

Xiaomei Wang, Qin Qi, Yuanyuan Wang, Huangan Wu, Xiaoming Jin, Huan Yao, Duiyin Jin, Yanan Liu, Cun Wang

Research output: Contribution to journalArticle

Abstract

Background: The pathogenesis of irritable bowel syndrome (IBS) is closely related to intestinal dysbacteriosis and can be controlled by moxibustion treatment. However, the mechanism underlying the therapeutic value of moxibustion in IBS treatment remains unknown. Methods: An IBS rat model was established by colorectal distention (CRD) stimulus and mustard oil clyster. Sixty-five male rats were randomly divided into six groups: normal, IBS model, moxibustion, electroacupuncture (EA), Bifid-triple Viable Capsule (BTVC) and Pinaverium Bromide (PB) groups. The moxibustion group was treated with mild moxibustion at the bilateral Tianshu (ST25) and Shangjuxu (ST37) for 10 min/day for 7 days, the EA group was given EA at ST25 and ST37 once daily for 7 days, while the BTVC group and PB groups received Bifid-triple Viable Capsule and Pinaverium Bromide solution (at the proportion of 1:0.018) respectively by gavage once daily for 7 days. After the treatment, abdominal withdrawal reflex (AWR) scores were determined based on CRD stimulus, gut microbiota profiling was conducted by 16S rRNA high-throughput sequencing. Results: Irritable bowel syndrome model rats had significantly increased AWR scores at all intensities (20, 40, 60 and 80 mmHg) compared with the normal group. Moxibustion treatment significantly reduced AWR scores compared with the IBS model group at all intensities. Across all groups the most abundant phyla were Bacteroidetes and Firmicutes followed by Proteobacteria and Candidatus Saccharibacteria. At genus level IBS model rats had a higher abundance of Prevotella, Bacteroides and Clostridium XI and a lower abundance of Lactobacillus and Clostridium XIVa compared with normal rats. These changes in microbiota profiles could however be reversed by moxibustion treatment. Alpha diversity was decreased in IBS model rats compared with normal rats, yet significantly increased in moxibustion- and PB-treated rats compared with IBS rats. Conclusion: Our findings suggest that moxibustion treats IBS by modulating the gut microbiota.

Original languageEnglish (US)
Article number63
JournalChinese Medicine (United Kingdom)
Volume13
Issue number1
DOIs
StatePublished - Dec 18 2018

Fingerprint

Moxibustion
Irritable Bowel Syndrome
Neurosciences
Health
Abdominal Reflex
Electroacupuncture
Capsules
Clostridium
Therapeutics
Dysbiosis
Gastrointestinal Microbiome
Prevotella
Bacteroidetes
Proteobacteria
Bacteroides
Microbiota
Lactobacillus

Keywords

  • 16S rRNA
  • Gut microbiota
  • Irritable bowel syndrome
  • Moxibustion

ASJC Scopus subject areas

  • Pharmacology
  • Complementary and alternative medicine

Cite this

Gut microbiota was modulated by moxibustion stimulation in rats with irritable bowel syndrome 11 Medical and Health Sciences 1109 Neurosciences. / Wang, Xiaomei; Qi, Qin; Wang, Yuanyuan; Wu, Huangan; Jin, Xiaoming; Yao, Huan; Jin, Duiyin; Liu, Yanan; Wang, Cun.

In: Chinese Medicine (United Kingdom), Vol. 13, No. 1, 63, 18.12.2018.

Research output: Contribution to journalArticle

Wang, Xiaomei ; Qi, Qin ; Wang, Yuanyuan ; Wu, Huangan ; Jin, Xiaoming ; Yao, Huan ; Jin, Duiyin ; Liu, Yanan ; Wang, Cun. / Gut microbiota was modulated by moxibustion stimulation in rats with irritable bowel syndrome 11 Medical and Health Sciences 1109 Neurosciences. In: Chinese Medicine (United Kingdom). 2018 ; Vol. 13, No. 1.
@article{7014b7dcfd88470d931841a1e9f0b255,
title = "Gut microbiota was modulated by moxibustion stimulation in rats with irritable bowel syndrome 11 Medical and Health Sciences 1109 Neurosciences",
abstract = "Background: The pathogenesis of irritable bowel syndrome (IBS) is closely related to intestinal dysbacteriosis and can be controlled by moxibustion treatment. However, the mechanism underlying the therapeutic value of moxibustion in IBS treatment remains unknown. Methods: An IBS rat model was established by colorectal distention (CRD) stimulus and mustard oil clyster. Sixty-five male rats were randomly divided into six groups: normal, IBS model, moxibustion, electroacupuncture (EA), Bifid-triple Viable Capsule (BTVC) and Pinaverium Bromide (PB) groups. The moxibustion group was treated with mild moxibustion at the bilateral Tianshu (ST25) and Shangjuxu (ST37) for 10 min/day for 7 days, the EA group was given EA at ST25 and ST37 once daily for 7 days, while the BTVC group and PB groups received Bifid-triple Viable Capsule and Pinaverium Bromide solution (at the proportion of 1:0.018) respectively by gavage once daily for 7 days. After the treatment, abdominal withdrawal reflex (AWR) scores were determined based on CRD stimulus, gut microbiota profiling was conducted by 16S rRNA high-throughput sequencing. Results: Irritable bowel syndrome model rats had significantly increased AWR scores at all intensities (20, 40, 60 and 80 mmHg) compared with the normal group. Moxibustion treatment significantly reduced AWR scores compared with the IBS model group at all intensities. Across all groups the most abundant phyla were Bacteroidetes and Firmicutes followed by Proteobacteria and Candidatus Saccharibacteria. At genus level IBS model rats had a higher abundance of Prevotella, Bacteroides and Clostridium XI and a lower abundance of Lactobacillus and Clostridium XIVa compared with normal rats. These changes in microbiota profiles could however be reversed by moxibustion treatment. Alpha diversity was decreased in IBS model rats compared with normal rats, yet significantly increased in moxibustion- and PB-treated rats compared with IBS rats. Conclusion: Our findings suggest that moxibustion treats IBS by modulating the gut microbiota.",
keywords = "16S rRNA, Gut microbiota, Irritable bowel syndrome, Moxibustion",
author = "Xiaomei Wang and Qin Qi and Yuanyuan Wang and Huangan Wu and Xiaoming Jin and Huan Yao and Duiyin Jin and Yanan Liu and Cun Wang",
year = "2018",
month = "12",
day = "18",
doi = "10.1186/s13020-018-0220-y",
language = "English (US)",
volume = "13",
journal = "Chinese Medicine",
issn = "1749-8546",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Gut microbiota was modulated by moxibustion stimulation in rats with irritable bowel syndrome 11 Medical and Health Sciences 1109 Neurosciences

AU - Wang, Xiaomei

AU - Qi, Qin

AU - Wang, Yuanyuan

AU - Wu, Huangan

AU - Jin, Xiaoming

AU - Yao, Huan

AU - Jin, Duiyin

AU - Liu, Yanan

AU - Wang, Cun

PY - 2018/12/18

Y1 - 2018/12/18

N2 - Background: The pathogenesis of irritable bowel syndrome (IBS) is closely related to intestinal dysbacteriosis and can be controlled by moxibustion treatment. However, the mechanism underlying the therapeutic value of moxibustion in IBS treatment remains unknown. Methods: An IBS rat model was established by colorectal distention (CRD) stimulus and mustard oil clyster. Sixty-five male rats were randomly divided into six groups: normal, IBS model, moxibustion, electroacupuncture (EA), Bifid-triple Viable Capsule (BTVC) and Pinaverium Bromide (PB) groups. The moxibustion group was treated with mild moxibustion at the bilateral Tianshu (ST25) and Shangjuxu (ST37) for 10 min/day for 7 days, the EA group was given EA at ST25 and ST37 once daily for 7 days, while the BTVC group and PB groups received Bifid-triple Viable Capsule and Pinaverium Bromide solution (at the proportion of 1:0.018) respectively by gavage once daily for 7 days. After the treatment, abdominal withdrawal reflex (AWR) scores were determined based on CRD stimulus, gut microbiota profiling was conducted by 16S rRNA high-throughput sequencing. Results: Irritable bowel syndrome model rats had significantly increased AWR scores at all intensities (20, 40, 60 and 80 mmHg) compared with the normal group. Moxibustion treatment significantly reduced AWR scores compared with the IBS model group at all intensities. Across all groups the most abundant phyla were Bacteroidetes and Firmicutes followed by Proteobacteria and Candidatus Saccharibacteria. At genus level IBS model rats had a higher abundance of Prevotella, Bacteroides and Clostridium XI and a lower abundance of Lactobacillus and Clostridium XIVa compared with normal rats. These changes in microbiota profiles could however be reversed by moxibustion treatment. Alpha diversity was decreased in IBS model rats compared with normal rats, yet significantly increased in moxibustion- and PB-treated rats compared with IBS rats. Conclusion: Our findings suggest that moxibustion treats IBS by modulating the gut microbiota.

AB - Background: The pathogenesis of irritable bowel syndrome (IBS) is closely related to intestinal dysbacteriosis and can be controlled by moxibustion treatment. However, the mechanism underlying the therapeutic value of moxibustion in IBS treatment remains unknown. Methods: An IBS rat model was established by colorectal distention (CRD) stimulus and mustard oil clyster. Sixty-five male rats were randomly divided into six groups: normal, IBS model, moxibustion, electroacupuncture (EA), Bifid-triple Viable Capsule (BTVC) and Pinaverium Bromide (PB) groups. The moxibustion group was treated with mild moxibustion at the bilateral Tianshu (ST25) and Shangjuxu (ST37) for 10 min/day for 7 days, the EA group was given EA at ST25 and ST37 once daily for 7 days, while the BTVC group and PB groups received Bifid-triple Viable Capsule and Pinaverium Bromide solution (at the proportion of 1:0.018) respectively by gavage once daily for 7 days. After the treatment, abdominal withdrawal reflex (AWR) scores were determined based on CRD stimulus, gut microbiota profiling was conducted by 16S rRNA high-throughput sequencing. Results: Irritable bowel syndrome model rats had significantly increased AWR scores at all intensities (20, 40, 60 and 80 mmHg) compared with the normal group. Moxibustion treatment significantly reduced AWR scores compared with the IBS model group at all intensities. Across all groups the most abundant phyla were Bacteroidetes and Firmicutes followed by Proteobacteria and Candidatus Saccharibacteria. At genus level IBS model rats had a higher abundance of Prevotella, Bacteroides and Clostridium XI and a lower abundance of Lactobacillus and Clostridium XIVa compared with normal rats. These changes in microbiota profiles could however be reversed by moxibustion treatment. Alpha diversity was decreased in IBS model rats compared with normal rats, yet significantly increased in moxibustion- and PB-treated rats compared with IBS rats. Conclusion: Our findings suggest that moxibustion treats IBS by modulating the gut microbiota.

KW - 16S rRNA

KW - Gut microbiota

KW - Irritable bowel syndrome

KW - Moxibustion

UR - http://www.scopus.com/inward/record.url?scp=85058797795&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85058797795&partnerID=8YFLogxK

U2 - 10.1186/s13020-018-0220-y

DO - 10.1186/s13020-018-0220-y

M3 - Article

VL - 13

JO - Chinese Medicine

JF - Chinese Medicine

SN - 1749-8546

IS - 1

M1 - 63

ER -