H3K4me1 marks DNA regions hypomethylated during aging in human stem and differentiated cells

Agustín F. Fernández, Gustavo F. Bayón, Rocío G. Urdinguio, Estela G. Toraño, María G. García, Antonella Carella, Sandra Petrus-Reurer, Cecilia Ferrero, Pablo Martinez-Camblor, Isabel Cubillo, Javier García-Castro, Jesus Delgado-Calle, Flor M. Pérez-Campo, José A. Riancho, Clara Bueno, Pablo Menéndez, Anouk Mentink, Katia Mareschi, Fabian Claire, Corrado FagnaniEmanuela Medda, Virgilia Toccaceli, Sonia Brescianini, Sebastián Moran, Manel Esteller, Alexandra Stolzing, Jan De Boer, Lorenza Nistico, Maria A. Stazi, Mario F. Fraga

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

In differentiated cells, aging is associated with hypermethylation of DNA regions enriched in repressive histone posttranslational modifications. However, the chromatin marks associated with changes in DNA methylation in adult stem cells during lifetime are still largely unknown. Here, DNA methylation profiling of mesenchymal stem cells (MSCs) obtained from individuals aged 2 to 92 yr identified 18,735 hypermethylated and 45,407 hypomethylated CpG sites associated with aging. As in differentiated cells, hypermethylated sequences were enriched in chromatin repressive marks. Most importantly, hypomethylated CpG sites were strongly enriched in the active chromatin mark H3K4me1 in stem and differentiated cells, suggesting this is a cell type-independent chromatin signature ofDNA hypomethylation during aging. Analysis of scedasticity showed that interindividual variability of DNA methylation increased during aging in MSCs and differentiated cells, providing a new avenue for the identification of DNA methylation changes over time. DNA methylation profiling of genetically identical individuals showed that both the tendency of DNA methylation changes and scedasticity depended on nongenetic as well as genetic factors. Our results indicate that the dynamics of DNA methylation during aging depend on a complex mixture of factors that include the DNA sequence, cell type, and chromatin context involved and that, depending on the locus, the changes can be modulated by genetic and/or external factors.

Original languageEnglish (US)
Pages (from-to)27-40
Number of pages14
JournalGenome Research
Volume25
Issue number1
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Fingerprint

DNA Methylation
Stem Cells
Chromatin
DNA
DNA Fingerprinting
Mesenchymal Stromal Cells
Histone Code
Adult Stem Cells
Cell Aging
Post Translational Protein Processing
Complex Mixtures

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Fernández, A. F., Bayón, G. F., Urdinguio, R. G., Toraño, E. G., García, M. G., Carella, A., ... Fraga, M. F. (2015). H3K4me1 marks DNA regions hypomethylated during aging in human stem and differentiated cells. Genome Research, 25(1), 27-40. https://doi.org/10.1101/gr.169011.113

H3K4me1 marks DNA regions hypomethylated during aging in human stem and differentiated cells. / Fernández, Agustín F.; Bayón, Gustavo F.; Urdinguio, Rocío G.; Toraño, Estela G.; García, María G.; Carella, Antonella; Petrus-Reurer, Sandra; Ferrero, Cecilia; Martinez-Camblor, Pablo; Cubillo, Isabel; García-Castro, Javier; Delgado-Calle, Jesus; Pérez-Campo, Flor M.; Riancho, José A.; Bueno, Clara; Menéndez, Pablo; Mentink, Anouk; Mareschi, Katia; Claire, Fabian; Fagnani, Corrado; Medda, Emanuela; Toccaceli, Virgilia; Brescianini, Sonia; Moran, Sebastián; Esteller, Manel; Stolzing, Alexandra; De Boer, Jan; Nistico, Lorenza; Stazi, Maria A.; Fraga, Mario F.

In: Genome Research, Vol. 25, No. 1, 01.01.2015, p. 27-40.

Research output: Contribution to journalArticle

Fernández, AF, Bayón, GF, Urdinguio, RG, Toraño, EG, García, MG, Carella, A, Petrus-Reurer, S, Ferrero, C, Martinez-Camblor, P, Cubillo, I, García-Castro, J, Delgado-Calle, J, Pérez-Campo, FM, Riancho, JA, Bueno, C, Menéndez, P, Mentink, A, Mareschi, K, Claire, F, Fagnani, C, Medda, E, Toccaceli, V, Brescianini, S, Moran, S, Esteller, M, Stolzing, A, De Boer, J, Nistico, L, Stazi, MA & Fraga, MF 2015, 'H3K4me1 marks DNA regions hypomethylated during aging in human stem and differentiated cells', Genome Research, vol. 25, no. 1, pp. 27-40. https://doi.org/10.1101/gr.169011.113
Fernández AF, Bayón GF, Urdinguio RG, Toraño EG, García MG, Carella A et al. H3K4me1 marks DNA regions hypomethylated during aging in human stem and differentiated cells. Genome Research. 2015 Jan 1;25(1):27-40. https://doi.org/10.1101/gr.169011.113
Fernández, Agustín F. ; Bayón, Gustavo F. ; Urdinguio, Rocío G. ; Toraño, Estela G. ; García, María G. ; Carella, Antonella ; Petrus-Reurer, Sandra ; Ferrero, Cecilia ; Martinez-Camblor, Pablo ; Cubillo, Isabel ; García-Castro, Javier ; Delgado-Calle, Jesus ; Pérez-Campo, Flor M. ; Riancho, José A. ; Bueno, Clara ; Menéndez, Pablo ; Mentink, Anouk ; Mareschi, Katia ; Claire, Fabian ; Fagnani, Corrado ; Medda, Emanuela ; Toccaceli, Virgilia ; Brescianini, Sonia ; Moran, Sebastián ; Esteller, Manel ; Stolzing, Alexandra ; De Boer, Jan ; Nistico, Lorenza ; Stazi, Maria A. ; Fraga, Mario F. / H3K4me1 marks DNA regions hypomethylated during aging in human stem and differentiated cells. In: Genome Research. 2015 ; Vol. 25, No. 1. pp. 27-40.
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AU - Bayón, Gustavo F.

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AU - Toraño, Estela G.

AU - García, María G.

AU - Carella, Antonella

AU - Petrus-Reurer, Sandra

AU - Ferrero, Cecilia

AU - Martinez-Camblor, Pablo

AU - Cubillo, Isabel

AU - García-Castro, Javier

AU - Delgado-Calle, Jesus

AU - Pérez-Campo, Flor M.

AU - Riancho, José A.

AU - Bueno, Clara

AU - Menéndez, Pablo

AU - Mentink, Anouk

AU - Mareschi, Katia

AU - Claire, Fabian

AU - Fagnani, Corrado

AU - Medda, Emanuela

AU - Toccaceli, Virgilia

AU - Brescianini, Sonia

AU - Moran, Sebastián

AU - Esteller, Manel

AU - Stolzing, Alexandra

AU - De Boer, Jan

AU - Nistico, Lorenza

AU - Stazi, Maria A.

AU - Fraga, Mario F.

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